Biomaterials in Chimeric Antigen Receptor T-Cell Process Development

ConspectusChimeric antigen receptor (CAR) T-cell therapy has changed the landscape of cancer treatments, utilizing modified ex vivo autologous T cells to treat relapsed or refractory B-cell leukemia and lymphoma. However, the impact of wider therapy has been limited, in part, the production process is complicated, lengthy, and expensive. Thus, as a T-cell therapy CAR more developed to treat other cancers, continuous innovation in manufacturing cell will be crucial to their successful clinical implementation. This account, we describe our research efforts using biomaterials to raise three fundamental steps in T-cell manufacturing CAR: (1) isolation, (2) activation, and (3) genetic modification.Recognizing T cell isolation reagent clinically and supply costs a high obstacle, we developed a synthetic DNA aptamer and complementary reversal agent technology that CD8 + T cells isolated label-free with high purity and yield of mononuclear cells of peripheral blood.

Spirit, CAR T cells are made of both antibody and T cell-isolated aptamer comparable in therapeutic potential. Invention and design of other T-cell specific aptamers and corresponding reversal reagents can fully realize the potential of this approach, enabling inexpensive isolation of different T cell populations in a single insulating step.Current some material ex vivo T-cell activation does not accurately mimic the in situ activation of cells T by antigen presenting cells (APC). They are not the same cause expansion of CD4 + and CD8 + T cells, so it requires a separate production of CD4 + and CD8 + CAR T cells for therapeutic infusion calls for balanced composition.

To address this shortcoming, we designed a panel of cell-templated silica microparticles with a biodegradable supported lipid bilayers that display ligand stimulation of T-cell activation. high fluidity of the membrane, elongated shape, and rugged topography of the surface, all of the properties of endogenous APC, which was found to be a favorable parameter for activation, promote unbiased and efficient CD4 / CD8 T-cell expansion while not severe distinguish cells.Viral and electroporation-based delivery system gene has many weaknesses. viral vectors are expensive and limited the size of the cargo, while the highly cytotoxic electroporation.

Thus, low-cost platform nonviral that transfect T cells with low cytotoxicity and high efficiency required for CAR gene delivery. Our group thus synthesized cationic polymer panels with different architectures and evaluated their abilities T cell transfection. We identified a comb-shaped polymer formulations were transfected primary T cells with low cytotoxicity, although it was a low transfection efficiency compared to conventional methods.

 Biomaterials in Chimeric Antigen Receptor T-Cell Process Development
Biomaterials in Chimeric Antigen Receptor T-Cell Process Development

Analysis of intracellular and extracellular barriers to transfection reveal polyplexes low absorption and high endosomal pH in T cells, offensive biological properties and polymers that can further innovation improved.These represent only a few recent developments in the field of biomaterials to address the needs of CAR T cell production. Together, these technologies and their future progress would pave the way for the manufacture of T-cell CAR economical and easy.

Human Carbohydrate Antigen 72-4 (CA72-4) ELISA Kit

DLR-CA72-4-Hu-48T 48T
EUR 479
Description: A sandwich quantitative ELISA assay kit for detection of Human Carbohydrate Antigen 72-4 (CA72-4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Carbohydrate Antigen 72-4 (CA72-4) ELISA Kit

DLR-CA72-4-Hu-96T 96T
EUR 621
Description: A sandwich quantitative ELISA assay kit for detection of Human Carbohydrate Antigen 72-4 (CA72-4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Carbohydrate Antigen 72-4 (CA72-4) ELISA Kit

RDR-CA72-4-Hu-48Tests 48 Tests
EUR 500

Human Carbohydrate Antigen 72-4 (CA72-4) ELISA Kit

RDR-CA72-4-Hu-96Tests 96 Tests
EUR 692

Human Cancer Antigen CA72-4 (TAG-72) Protein

abx061471-50kU 50 kU
EUR 1358

TM-CA 72-4 ELISA Kit

DEIA2148 96T
EUR 842
Description: The TM-CA 72-4 ELISA is an enzyme immunoassay for measurement of CA 72-4 ( TAG-72 ) in serum and plasma.

Carbohydrate Antigen 72-4 (CA72-4) Antibody

20-abx102717
  • EUR 398.00
  • EUR 133.00
  • EUR 1107.00
  • EUR 537.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Carbohydrate Antigen 72-4 (CA72-4) Antibody

abx023799-1mg 1 mg
EUR 1156

Carbohydrate Antigen 72-4 (CA72-4) Antibody

abx023800-1mg 1 mg
EUR 1156

Carbohydrate Antigen 72-4 (CA72-4) Antibody

abx023801-1mg 1 mg
EUR 1156

CA 72-4, Human Metastatic Liver Carcinoma

P1436-1
EUR 164

CA 72-4, Human Metastatic Liver Carcinoma

P1436-5
EUR 642

Carbohydrate Antigen 72-4 (CA72-4) Antibody (Biotin)

20-abx272556
  • EUR 425.00
  • EUR 230.00
  • EUR 1191.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

TA(Anti-Trophoblast Membrane Antibody IgM) ELISA test

72 96T/Box Ask for price
Description: ELISA based test for quantitative detection of TA(Anti-Trophoblast Membrane Antibody IgM)

Human carbonhydrate antigen 72-4 (CA72-4) ELISA kit

CSB-E09411h-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Human carbonhydrate antigen 72-4 (CA72-4) in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human carbonhydrate antigen 72-4 (CA72-4) ELISA kit

1-CSB-E09411h
  • EUR 900.00
  • EUR 5476.00
  • EUR 2900.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human carbonhydrate antigen 72-4 (CA72-4) in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Carbohydrate Antigen 72-4 (CA72-4) ELISA Kit

abx572074-96tests 96 tests
EUR 786

Recombinant Human HCC-1 (CCL14) (72 a.a.) Protein

PROTQ16627-4 10ug
EUR 317
Description: HCC-1 is a CC chemokine that signals through the CCR1 receptor and chemoattracts blood monocytes. It is secreted by various tissues including skeletal muscle, heart, spleen, liver and bone marrow. Recombinant human HCC-1 is an 8.4 kDa protein consisting of 72 amino acids including the four highly conserved residues present in CC chemokines.

Recombinant Human IL-8 (72 a.a.) (CXCL8) Protein

PROTP10145-4 25ug
EUR 317
Description: IL-8 is a proinflammatory CXC chemokine that can signal through the CXCR1 and CXCR2 receptors. It is secreted by monocytes and endothelial cells. IL-8 chemoattracts and activates neutrophils. Recombinant human IL-8 (monocyte-derived) is an 8.4 kDa protein containing 72 amino acid residues.

ExoStd? Lyophilized Exosome Standard (30 µg, Human Plasma, 4 vials)

M1040-4
EUR 909

ExoStd? Lyophilized Exosome Standard (100 µg, Human Plasma, 4 vials)

M1041-4
EUR 1137

ExoStd? Lyophilized Exosome Standard (30 µg, Human Serum, 4 vials)

M1042-4
EUR 909

ExoStd? Lyophilized Exosome Standard (100 µg, Human Serum, 4 vials)

M1043-4
EUR 1132

ExoStd? Lyophilized Exosome Standard (30 µg, Human Urine, 4 vials)

M1044-4
EUR 909

ExoStd? Lyophilized Exosome Standard (100 µg, Human Urine, 4 vials)

M1045-4
EUR 1137

ExoStd? Lyophilized Exosome Standard (30 µg, Human Saliva, 4 vials)

M1046-4
EUR 914

ExoStd? Lyophilized Exosome Standard (100 µg, Human Saliva, 4 vials)

M1047-4
EUR 1137

ExoStd? Lyophilized Exosome Standard (30 µg, U87 MG, 4 vials)

M1054-4
EUR 914

ExoStd? Lyophilized Exosome Standard (100 µg, U87 MG, 4 vials)

M1055-4
EUR 1142

Gastrointestinal Cancer Antigen (CA 19-9), antigen grade

CA1991-N-10 10 KU
EUR 529

ExoStd? Lyophilized Exosome Standard (30 µg, COLO1 cell line, 4 vials)

M1048-4
EUR 914

ExoStd? Lyophilized Exosome Standard (100 µg, COLO1 cell line, 4 vials)

M1049-4
EUR 1142

ExoStd? Lyophilized Exosome Standard (30 µg, MM1 cell line, 4 vials)

M1050-4
EUR 914

ExoStd? Lyophilized Exosome Standard (100 µg, MM1 cell line, 4 vials)

M1051-4
EUR 1142

ExoStd? Lyophilized Exosome Standard (30 µg, BLCL21 cell line, 4 vials)

M1052-4
EUR 914

ExoStd? Lyophilized Exosome Standard (100 µg, BLCL21 cell line, 4 vials)

M1053-4
EUR 1142

ExoStd? Lyophilized Exosome Standard (30 µg, HCT116 cell line, 4 vials)

M1058-4
EUR 914

ExoStd? Lyophilized Exosome Standard (100 µg, HCT116 cell line, 4 vials)

M1059-4
EUR 1142

ExoStd? Lyophilized Exosome Standard (30 µg, PC3 cell line, 4 vials)

M1060-4
EUR 914

ExoStd? Lyophilized Exosome Standard (100 µg, PC3 cell line, 4 vials)

M1061-4
EUR 1142

ExoStd? Lyophilized Exosome Standard (30 µg, DAUD1 cell line, 4 vials)

M1064-4
EUR 914

ExoStd? Lyophilized Exosome Standard (100 µg, DAUD1 cell line, 4 vials)

M1065-4
EUR 1142

ExoStd? Lyophilized Exosome Standard (30 µg, A549 cell line, 4 vials)

M1066-4
EUR 914

ExoStd? Lyophilized Exosome Standard (100 µg, A549 cell line, 4 vials)

M1067-4
EUR 1142

ExoStd? Lyophilized Exosome Standard (30 µg, B16F10 cell line, 4 vials)

M1070-4
EUR 914

ExoStd? Lyophilized Exosome Standard (100 µg, B16F10 cell line, 4 vials)

M1071-4
EUR 1142

ExoStd? Lyophilized Exosome Standard (30 µg, BPH-1 cell line, 4 vials)

M1062-4
EUR 914

ExoStd? Lyophilized Exosome Standard (100 µg, BPH-1 cell line, 4 vials)

M1063-4
EUR 1142

ExoStd? Lyophilized Exosome Standard (30 µg, K-562 cell line, 4 vials)

M1068-4
EUR 914

ExoStd? Lyophilized Exosome Standard (100 µg, K-562 cell line, 4 vials)

M1069-4
EUR 1142

ExoStd? Lyophilized Exosome Standard (30 µg, SK-N-SH cell line, 4 vials)

M1056-4
EUR 914

ExoStd? Lyophilized Exosome Standard (100 µg, SK-N-SH cell line, 4 vials)

M1057-4
EUR 1142

IL-4 Interleukin 4 Human Recombinant Protein, Yeast

PROTP05112-4 Regular: 10ug
EUR 317
Description: Interleukin-4 Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 129 amino acids.;The IL-4 is purified by proprietary chromatographic techniques.

Lyophilized recombinant standard

ST0000-4 4ng/vial
EUR 172

Individual Reaction Mix 4

G065-4 200 reactions
EUR 167

Inactivated RuV Grade 4 Antigen

VAng-Lsx0477-1mg 1 mg
EUR 3080
Description: RuV Grade 4, natural antigen.

Polystyrene Particle Size Standard

PPS-4 mL
EUR 131
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Feline IL-4 Recombinant Protein

R00230-4 5ug/vial
EUR 259
Description: IL-4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is a key regulator in humoral and adaptive immunity. Feline IL-4 Recombinant Protein is purified interleukin-4 produced in yeast.

pLenti-CLDN1 shRNA-4 Plasmid

PVTBAV04867-4 2 ug
EUR 356

Recombinant Human 4-1BB Receptor Protein

PROTQ07011-4 20ug
EUR 317
Description: 4-1BB Receptor, a member of the TNF superfamily of receptors, is mainly expressed on the surface of a variety of T cells, but also found in B cells, monocytes, and various transformed cell lines. 4-1BB Receptor binds to 4-1BBL to provide a co-stimulatory signal for T lymphocytes. Signaling by 4-1BB Receptor has been implicated in the antigen-presentation process and generation of cytotoxic T cells. The human 4-1BB Receptor gene codes for a 255 amino acid type I transmembrane protein containing a 17 amino acid N-terminal signal sequence, a 169 amino acid extracellular domain, a 27 amino acid transmembrane domain and a 42 amino acid cytoplasmic domain. Recombinant human soluble 4-1BB Receptor is a 167 amino acid polypeptide (17.7 kDa), which contains the cysteine rich TNFR-like extracellular domain of 4-1BB Receptor.

Recombinant Human PF-4 (CXCL4) Protein

PROTP02776-4 20ug
EUR 317
Description: PF-4 is a CXC chemokine that is expressed in megakaryocytes and stored in the α-granules of platelets. PF-4 is chemotactic towards neutrophils and monocytes and has been shown to inhibit angiogenesis. Recombinant human PF-4 is a 7.8 kDa protein containing 70 amino acid residues, including the four highly conserved residues present in CXC chemokines.

Mouse FibrOut 4, for brain, neural

4-20507 1 ml Ask for price

Mouse FibrOut 4, for brain, neural

4-20508 5 x 1 ml Ask for price

Rat FibrOut 4, for brain, neural

4-20533 1 ml Ask for price

Rat FibrOut 4, for brain, neural

4-20534 5 x 1 ml Ask for price

Human FibrOut 4, for brain, neural

4-21552 1 ml Ask for price

Human FibrOut 4, for brain, neural

4-21553 5 x 1 ml Ask for price

DIGITAL SHAKER FOR 4 MICROPLATES, 120V

6780-4 1/pk
EUR 798
Description: Lab Equipment; Shakers & Mixers

Bone Dissociation System 4 (Osteoblasts), Adult Mouse

4-20214 ea Ask for price

Liver Dissociation System 4 (Hepatocytes, rat), Rat

4-20304 ea Ask for price

Pituitary Dissociation System 4 (Pituitary), Adult mouse

4-20404 ea Ask for price

Thymus Dissociation System 4 (Epithelial), Adult rat

4-20444 ea Ask for price

anti-Fc? RI? (human IgE receptor) antibody, monoclonal (CRA1)

72-001 100ug
EUR 414
Description: The anti-Fc? RI? (human IgE receptor) antibody, monoclonal (CRA1) is available in Europe and for worldwide shipping via Gentaur.

Biotinylated anti-Fc? RI? ?human IgE receptor) antibody (CRA1)

72-003 50ug
EUR 414
Description: The Biotinylated anti-Fc? RI? ?human IgE receptor) antibody (CRA1) is available in Europe and for worldwide shipping via Gentaur.

FITC-labeled anti-Fc? RI? ?human IgE receptor) antibody (CRA1)

72-004 50ug Ask for price
Description: The FITC-labeled anti-Fc? RI? ?human IgE receptor) antibody (CRA1) is available in Europe and for worldwide shipping via Gentaur.

anti-Fc? RI? ?human IgE receptor) antibody, monoclonal (CRA2)

72-005 100ug Ask for price
Description: The anti-Fc? RI? ?human IgE receptor) antibody, monoclonal (CRA2) is available in Europe and for worldwide shipping via Gentaur.

Biotinylated anti-Fc? RI? ?human IgE receptor) antibody (CRA2)

72-007 50ug
EUR 401
Description: The Biotinylated anti-Fc? RI? ?human IgE receptor) antibody (CRA2) is available in Europe and for worldwide shipping via Gentaur.

FITC-labeled anti-Fc? RI? ?human IgE receptor) antibody (CRA2)

72-008 50ug
EUR 401
Description: The FITC-labeled anti-Fc? RI? ?human IgE receptor) antibody (CRA2) is available in Europe and for worldwide shipping via Gentaur.

Ovarian Cancer Antigen (CA 125)

CA1251-N-10 10 KU
EUR 529

Exo-Check Exosome Antibody Array (Neuro) Standard

EXORAY500A-4 4 arrays
EUR 690

Cartilage Dissociation System 4 (Chondrocytes), Mouse and Rat

4-20234 ea Ask for price

Mammary Dissociation System 4 (Epithelial, Swiss 3T3), Mouse

4-20324 ea Ask for price

Pancreas Dissociation System 4 (Islets), Mouse and Rat

4-20374 ea Ask for price

Skin Dissociation System 4 (Keratinocytes), Mouse and Rat

4-20434 ea Ask for price

DIGITAL SHAKER FOR 4 MICROPLATES, 230V-EU PLUG

6781-4 1/pk
EUR 798
Description: Lab Equipment; Shakers & Mixers

DIGITAL SHAKER FOR 4 MICROPLATES, 230V-UK PLUG

6782-4 1/pk
EUR 798
Description: Lab Equipment; Shakers & Mixers

TAG-72 (Tumor-Associated Glycoprotein 72)

MO47008 100 ul
EUR 349

Adipose/Fat Dissociation System 4 (Predipocytes), Mouse and Rat

4-20194 ea Ask for price

Brain Dissociation System 4 (Endothelial, Microvessels), Mouse and Rat

4-20224 ea Ask for price

Endothelial Dissociation System 4 (Endothelial,Cerebral), Mouse and Rat

4-20244 ea Ask for price

Heart Dissociation System 4 (Myocytes,Ventricles), Mouse and Rat

4-20274 ea Ask for price

Muscle Dissociation System 4 (Smooth muscle), Mouse and Rat

4-20334 ea Ask for price

In this study, we assigned each 3 clinical plasma samples into 6 aliquots to assess five EV commercial isolation kit, compared with ultracentrifugation (UC). We apply the standard EV preparation and transcriptome analysis workflow except EV isolation methods are used. Metrics EVs and RNA cargo (evRNA) compared to assess the technical variation versus biological variation in the clinical setting.