Biomaterials in Chimeric Antigen Receptor T-Cell Process Development

ConspectusChimeric antigen receptor (CAR) T-cell therapy has changed the landscape of cancer treatments, utilizing modified ex vivo autologous T cells to treat relapsed or refractory B-cell leukemia and lymphoma. However, the impact of wider therapy has been limited, in part, the production process is complicated, lengthy, and expensive. Thus, as a T-cell therapy CAR more developed to treat other cancers, continuous innovation in manufacturing cell will be crucial to their successful clinical implementation. This account, we describe our research efforts using biomaterials to raise three fundamental steps in T-cell manufacturing CAR: (1) isolation, (2) activation, and (3) genetic modification.Recognizing T cell isolation reagent clinically and supply costs a high obstacle, we developed a synthetic DNA aptamer and complementary reversal agent technology that CD8 + T cells isolated label-free with high purity and yield of mononuclear cells of peripheral blood.

Spirit, CAR T cells are made of both antibody and T cell-isolated aptamer comparable in therapeutic potential. Invention and design of other T-cell specific aptamers and corresponding reversal reagents can fully realize the potential of this approach, enabling inexpensive isolation of different T cell populations in a single insulating step.Current some material ex vivo T-cell activation does not accurately mimic the in situ activation of cells T by antigen presenting cells (APC). They are not the same cause expansion of CD4 + and CD8 + T cells, so it requires a separate production of CD4 + and CD8 + CAR T cells for therapeutic infusion calls for balanced composition.

To address this shortcoming, we designed a panel of cell-templated silica microparticles with a biodegradable supported lipid bilayers that display ligand stimulation of T-cell activation. high fluidity of the membrane, elongated shape, and rugged topography of the surface, all of the properties of endogenous APC, which was found to be a favorable parameter for activation, promote unbiased and efficient CD4 / CD8 T-cell expansion while not severe distinguish cells.Viral and electroporation-based delivery system gene has many weaknesses. viral vectors are expensive and limited the size of the cargo, while the highly cytotoxic electroporation.

Thus, low-cost platform nonviral that transfect T cells with low cytotoxicity and high efficiency required for CAR gene delivery. Our group thus synthesized cationic polymer panels with different architectures and evaluated their abilities T cell transfection. We identified a comb-shaped polymer formulations were transfected primary T cells with low cytotoxicity, although it was a low transfection efficiency compared to conventional methods.

 Biomaterials in Chimeric Antigen Receptor T-Cell Process Development
Biomaterials in Chimeric Antigen Receptor T-Cell Process Development

Analysis of intracellular and extracellular barriers to transfection reveal polyplexes low absorption and high endosomal pH in T cells, offensive biological properties and polymers that can further innovation improved.These represent only a few recent developments in the field of biomaterials to address the needs of CAR T cell production. Together, these technologies and their future progress would pave the way for the manufacture of T-cell CAR economical and easy.

Human Carbohydrate Antigen 72-4 (CA72-4) ELISA Kit

RDR-CA72-4-Hu-48Tests 48 Tests
EUR 600

Human Carbohydrate Antigen 72-4 (CA72-4) ELISA Kit

RDR-CA72-4-Hu-96Tests 96 Tests
EUR 830.4

Human Carbohydrate Antigen 72-4 (CA72-4) ELISA Kit

RD-CA72-4-Hu-48Tests 48 Tests
EUR 573.6

Human Carbohydrate Antigen 72-4 (CA72-4) ELISA Kit

RD-CA72-4-Hu-96Tests 96 Tests
EUR 794.4

Human Cancer Antigen CA72-4 (TAG-72) Protein

abx061471-50kU 50 kU
EUR 1629.6

TM-CA 72-4 ELISA Kit

DEIA2148 96T
EUR 1010.4
Description: The TM-CA 72-4 ELISA is an enzyme immunoassay for measurement of CA 72-4 ( TAG-72 ) in serum and plasma.

Carbohydrate Antigen 72-4 (CA72-4) Antibody

20-abx102717
  • EUR 477.60
  • EUR 159.60
  • EUR 1328.40
  • EUR 644.40
  • EUR 376.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Carbohydrate Antigen 72-4 (CA72-4) Antibody

abx023799-1mg 1 mg
EUR 1387.2

Carbohydrate Antigen 72-4 (CA72-4) Antibody

abx023800-1mg 1 mg
EUR 1387.2

Carbohydrate Antigen 72-4 (CA72-4) Antibody

abx023801-1mg 1 mg
EUR 1387.2

CA 72-4, Human Metastatic Liver Carcinoma

P1436-1 each
EUR 196.8

CA 72-4, Human Metastatic Liver Carcinoma

P1436-5 each
EUR 770.4

Carbohydrate Antigen 72-4 (CA72-4) Antibody (Biotin)

20-abx272556
  • EUR 510.00
  • EUR 276.00
  • EUR 1429.20
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

TA(Anti-Trophoblast Membrane Antibody IgM) ELISA test

72 96T/Box Ask for price
Description: ELISA based test for quantitative detection of TA(Anti-Trophoblast Membrane Antibody IgM)

Human carbonhydrate antigen 72-4 (CA72-4) ELISA kit

CSB-E09411h-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human carbonhydrate antigen 72-4 (CA72-4) in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human carbonhydrate antigen 72-4 (CA72-4) ELISA kit

1-CSB-E09411h
  • EUR 1080.00
  • EUR 6571.20
  • EUR 3480.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human carbonhydrate antigen 72-4 (CA72-4) in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Carbohydrate Antigen 72-4 (CA72-4) ELISA Kit

abx572074-96tests 96 tests
EUR 943.2

ICP-MS Instrument Check Standard 4

CL-ICS-4 125ML
EUR 112.86

Recombinant Human HCC-1 (CCL14) (72 a.a.) Protein

PROTQ16627-4 10ug
EUR 380.4
Description: HCC-1 is a CC chemokine that signals through the CCR1 receptor and chemoattracts blood monocytes. It is secreted by various tissues including skeletal muscle, heart, spleen, liver and bone marrow. Recombinant human HCC-1 is an 8.4 kDa protein consisting of 72 amino acids including the four highly conserved residues present in CC chemokines.

Recombinant Human IL-8 (72 a.a.) (CXCL8) Protein

PROTP10145-4 25ug
EUR 380.4
Description: IL-8 is a proinflammatory CXC chemokine that can signal through the CXCR1 and CXCR2 receptors. It is secreted by monocytes and endothelial cells. IL-8 chemoattracts and activates neutrophils. Recombinant human IL-8 (monocyte-derived) is an 8.4 kDa protein containing 72 amino acid residues.

ExoStd? Lyophilized Exosome Standard (30 µg, Human Plasma, 4 vials)

M1040-4 each
EUR 1090.8

ExoStd? Lyophilized Exosome Standard (100 µg, Human Plasma, 4 vials)

M1041-4 each
EUR 1364.4

ExoStd? Lyophilized Exosome Standard (30 µg, Human Serum, 4 vials)

M1042-4 each
EUR 1090.8

ExoStd? Lyophilized Exosome Standard (100 µg, Human Serum, 4 vials)

M1043-4 each
EUR 1358.4

ExoStd? Lyophilized Exosome Standard (30 µg, Human Urine, 4 vials)

M1044-4 each
EUR 1090.8

ExoStd? Lyophilized Exosome Standard (100 µg, Human Urine, 4 vials)

M1045-4 each
EUR 1364.4

ExoStd? Lyophilized Exosome Standard (30 µg, Human Saliva, 4 vials)

M1046-4 each
EUR 1096.8

ExoStd? Lyophilized Exosome Standard (100 µg, Human Saliva, 4 vials)

M1047-4 each
EUR 1364.4

ExoStd? Lyophilized Exosome Standard (30 µg, U87 MG, 4 vials)

M1054-4 each
EUR 1096.8

ExoStd? Lyophilized Exosome Standard (100 µg, U87 MG, 4 vials)

M1055-4 each
EUR 1370.4

Gastrointestinal Cancer Antigen (CA 19-9), antigen grade

CA1991-N-10 10 KU
EUR 634.8

ExoStd? Lyophilized Exosome Standard (30 µg, COLO1 cell line, 4 vials)

M1048-4 each
EUR 1096.8

ExoStd? Lyophilized Exosome Standard (100 µg, COLO1 cell line, 4 vials)

M1049-4 each
EUR 1370.4

ExoStd? Lyophilized Exosome Standard (30 µg, MM1 cell line, 4 vials)

M1050-4 each
EUR 1096.8

ExoStd? Lyophilized Exosome Standard (100 µg, MM1 cell line, 4 vials)

M1051-4 each
EUR 1370.4

ExoStd? Lyophilized Exosome Standard (30 µg, BLCL21 cell line, 4 vials)

M1052-4 each
EUR 1096.8

ExoStd? Lyophilized Exosome Standard (100 µg, BLCL21 cell line, 4 vials)

M1053-4 each
EUR 1370.4

ExoStd? Lyophilized Exosome Standard (30 µg, HCT116 cell line, 4 vials)

M1058-4 each
EUR 1096.8

ExoStd? Lyophilized Exosome Standard (100 µg, HCT116 cell line, 4 vials)

M1059-4 each
EUR 1370.4

ExoStd? Lyophilized Exosome Standard (30 µg, PC3 cell line, 4 vials)

M1060-4 each
EUR 1096.8

ExoStd? Lyophilized Exosome Standard (100 µg, PC3 cell line, 4 vials)

M1061-4 each
EUR 1370.4

ExoStd? Lyophilized Exosome Standard (30 µg, DAUD1 cell line, 4 vials)

M1064-4 each
EUR 1096.8

ExoStd? Lyophilized Exosome Standard (100 µg, DAUD1 cell line, 4 vials)

M1065-4 each
EUR 1370.4

ExoStd? Lyophilized Exosome Standard (30 µg, A549 cell line, 4 vials)

M1066-4 each
EUR 1096.8

ExoStd? Lyophilized Exosome Standard (100 µg, A549 cell line, 4 vials)

M1067-4 each
EUR 1370.4

ExoStd? Lyophilized Exosome Standard (30 µg, B16F10 cell line, 4 vials)

M1070-4 each
EUR 1096.8

ExoStd? Lyophilized Exosome Standard (100 µg, B16F10 cell line, 4 vials)

M1071-4 each
EUR 1370.4

ExoStd? Lyophilized Exosome Standard (30 µg, BPH-1 cell line, 4 vials)

M1062-4 each
EUR 1096.8

ExoStd? Lyophilized Exosome Standard (100 µg, BPH-1 cell line, 4 vials)

M1063-4 each
EUR 1370.4

ExoStd? Lyophilized Exosome Standard (30 µg, K-562 cell line, 4 vials)

M1068-4 each
EUR 1096.8

ExoStd? Lyophilized Exosome Standard (100 µg, K-562 cell line, 4 vials)

M1069-4 each
EUR 1370.4

ExoStd? Lyophilized Exosome Standard (30 µg, SK-N-SH cell line, 4 vials)

M1056-4 each
EUR 1096.8

ExoStd? Lyophilized Exosome Standard (100 µg, SK-N-SH cell line, 4 vials)

M1057-4 each
EUR 1370.4

IL-4 Interleukin 4 Human Recombinant Protein, Yeast

PROTP05112-4 Regular: 10ug
EUR 380.4
Description: Interleukin-4 Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 129 amino acids.;The IL-4 is purified by proprietary chromatographic techniques.

50ml Adapter for S-4-72

E5804784006 PK2
EUR 182.4

Individual Reaction Mix 4

G065-4 200 reactions
EUR 200.4

Feline IL-4 Recombinant Protein

R00230-4 5ug/vial
EUR 310.8
Description: IL-4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is a key regulator in humoral and adaptive immunity. Feline IL-4 Recombinant Protein is purified interleukin-4 produced in yeast.

pLenti-CLDN1 shRNA-4 Plasmid

PVTBAV04867-4 2 ug
EUR 427.2

Digital Shaker For 4 Microplates

6782-4 EACH
EUR 1110.36

Inactivated RuV Grade 4 Antigen

VAng-Lsx0477-1mg 1 mg
EUR 3696
Description: RuV Grade 4, natural antigen.

Lyophilized recombinant standard

ST0000-4 4ng/vial
EUR 206.4

DIGITAL SHAKER FOR 4 MICROPLATES, 120V

6780-4 1/pk
EUR 957.6
Description: Lab Equipment; Shakers & Mixers

Mouse FibrOut 4, for brain, neural

4-20507 1 ml Ask for price

Mouse FibrOut 4, for brain, neural

4-20508 5 x 1 ml Ask for price

Rat FibrOut 4, for brain, neural

4-20533 1 ml Ask for price

Rat FibrOut 4, for brain, neural

4-20534 5 x 1 ml Ask for price

Human FibrOut 4, for brain, neural

4-21552 1 ml Ask for price

Human FibrOut 4, for brain, neural

4-21553 5 x 1 ml Ask for price

Recombinant Human 4-1BB Receptor Protein

PROTQ07011-4 20ug
EUR 380.4
Description: 4-1BB Receptor, a member of the TNF superfamily of receptors, is mainly expressed on the surface of a variety of T cells, but also found in B cells, monocytes, and various transformed cell lines. 4-1BB Receptor binds to 4-1BBL to provide a co-stimulatory signal for T lymphocytes. Signaling by 4-1BB Receptor has been implicated in the antigen-presentation process and generation of cytotoxic T cells. The human 4-1BB Receptor gene codes for a 255 amino acid type I transmembrane protein containing a 17 amino acid N-terminal signal sequence, a 169 amino acid extracellular domain, a 27 amino acid transmembrane domain and a 42 amino acid cytoplasmic domain. Recombinant human soluble 4-1BB Receptor is a 167 amino acid polypeptide (17.7 kDa), which contains the cysteine rich TNFR-like extracellular domain of 4-1BB Receptor.

Recombinant Human PF-4 (CXCL4) Protein

PROTP02776-4 20ug
EUR 380.4
Description: PF-4 is a CXC chemokine that is expressed in megakaryocytes and stored in the α-granules of platelets. PF-4 is chemotactic towards neutrophils and monocytes and has been shown to inhibit angiogenesis. Recombinant human PF-4 is a 7.8 kDa protein containing 70 amino acid residues, including the four highly conserved residues present in CXC chemokines.

ICP-MS Multi-Element Solution 4

CLMS-4 125ML
EUR 221.16

Rotor S-4-72 + 4x250ml round buckets

E5804746007 EACH
EUR 1997.28

Polystyrene Particle Size Standard

PPS-4 mL
EUR 157.2
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Bone Dissociation System 4 (Osteoblasts), Adult Mouse

4-20214 ea Ask for price

Liver Dissociation System 4 (Hepatocytes, rat), Rat

4-20304 ea Ask for price

Pituitary Dissociation System 4 (Pituitary), Adult mouse

4-20404 ea Ask for price

Thymus Dissociation System 4 (Epithelial), Adult rat

4-20444 ea Ask for price

anti-Fc? RI? (human IgE receptor) antibody, monoclonal (CRA1)

72-001 100ug
EUR 496.8
Description: The anti-Fc? RI? (human IgE receptor) antibody, monoclonal (CRA1) is available in Europe and for worldwide shipping via Gentaur.

Biotinylated anti-Fc? RI? ?human IgE receptor) antibody (CRA1)

72-003 50ug
EUR 496.8
Description: The Biotinylated anti-Fc? RI? ?human IgE receptor) antibody (CRA1) is available in Europe and for worldwide shipping via Gentaur.

FITC-labeled anti-Fc? RI? ?human IgE receptor) antibody (CRA1)

72-004 50ug Ask for price
Description: The FITC-labeled anti-Fc? RI? ?human IgE receptor) antibody (CRA1) is available in Europe and for worldwide shipping via Gentaur.

anti-Fc? RI? ?human IgE receptor) antibody, monoclonal (CRA2)

72-005 100ug Ask for price
Description: The anti-Fc? RI? ?human IgE receptor) antibody, monoclonal (CRA2) is available in Europe and for worldwide shipping via Gentaur.

Biotinylated anti-Fc? RI? ?human IgE receptor) antibody (CRA2)

72-007 50ug
EUR 481.2
Description: The Biotinylated anti-Fc? RI? ?human IgE receptor) antibody (CRA2) is available in Europe and for worldwide shipping via Gentaur.

FITC-labeled anti-Fc? RI? ?human IgE receptor) antibody (CRA2)

72-008 50ug
EUR 481.2
Description: The FITC-labeled anti-Fc? RI? ?human IgE receptor) antibody (CRA2) is available in Europe and for worldwide shipping via Gentaur.

Ovarian Cancer Antigen (CA 125)

CA1251-N-10 10 KU
EUR 634.8

DIGITAL SHAKER FOR 4 MICROPLATES, 230V-EU PLUG

6781-4 1/pk
EUR 957.6
Description: Lab Equipment; Shakers & Mixers

Cartilage Dissociation System 4 (Chondrocytes), Mouse and Rat

4-20234 ea Ask for price

Mammary Dissociation System 4 (Epithelial, Swiss 3T3), Mouse

4-20324 ea Ask for price

Pancreas Dissociation System 4 (Islets), Mouse and Rat

4-20374 ea Ask for price

Skin Dissociation System 4 (Keratinocytes), Mouse and Rat

4-20434 ea Ask for price

Pesticide Mix 4 Containing 15 Compounds in Acetonitrile

SPXPR-4 1ML
EUR 175.56

Adipose/Fat Dissociation System 4 (Predipocytes), Mouse and Rat

4-20194 ea Ask for price

Brain Dissociation System 4 (Endothelial, Microvessels), Mouse and Rat

4-20224 ea Ask for price

Endothelial Dissociation System 4 (Endothelial,Cerebral), Mouse and Rat

4-20244 ea Ask for price

Heart Dissociation System 4 (Myocytes,Ventricles), Mouse and Rat

4-20274 ea Ask for price

Muscle Dissociation System 4 (Smooth muscle), Mouse and Rat

4-20334 ea Ask for price

Parotid Dissociation System 4 (Parotid acinar), Mouse and Rat

4-20394 ea Ask for price

Cancer Antigen CA-125 Antigen (Human Fluids)

VAng-Wyb8639-inquire inquire Ask for price
Description: CA-125, Calibrator Grade, cancer antigen from Human Fluids, 53.26 KU/mL.

Cancer Antigen CA-125 Antigen (Human Adenocarcinoma)

VAng-Wyb8642-inquire inquire Ask for price
Description: CA-125, Calibrator Grade, Cancer antigen from Human Adenocarcinoma, 500 kIU / mL.

Exo-Check Exosome Antibody Array (Neuro) Standard

EXORAY500A-4 4 arrays
EUR 828

Groundwater and Wastewater Pollution Control Check Standard

MN-4 125ML
EUR 132.24

Epithelial Dissociation System 4 (Epithelial,Submandibular salivary), Mouse and Rat

4-20254 ea Ask for price

Heart Dissociation System 4 (Retinal pigment epithelial), Mouse and Rat

4-20294 ea Ask for price

Lung Dissociation System 4 (Alveolar type II), Mouse and Rat

4-20314 ea Ask for price

Reproductive Tissue Dissociation System 4 (Epithelial, vagina), Mouse and Rat

4-20414 ea Ask for price

TAG-72 (Tumor-Associated Glycoprotein 72)

MO47008 100 ul
EUR 418.8

Human Cancer OptiTDS1: Tissue Dissociation System

4-28024 1 Kit Ask for price

Human Cancer OptiTDS2: Tissue Dissociation System

4-28025 1 Kit Ask for price

Human Cancer OptiTDS3: Tissue Dissociation System

4-28026 1 Kit Ask for price

Human Cancer OptiTDS4: Tissue Dissociation System

4-28027 1 Kit Ask for price

Human Cancer OptiTDS5: Tissue Dissociation System

4-28028 1 Kit Ask for price

Human Cancer OptiTDS6: Tissue Dissociation System

4-28029 1 Kit Ask for price

Human Cancer OptiTDS7: Tissue Dissociation System

4-28030 1 Kit Ask for price

Human Cancer OptiTDS8: Tissue Dissociation System

4-28031 1 Kit Ask for price

Human Cancer OptiTDS9: Tissue Dissociation System

4-28032 1 Kit Ask for price

Human Cancer OptiTDS10: Tissue Dissociation System

4-28033 1 Kit Ask for price

Human Cancer OptiTDS11: Tissue Dissociation System

4-28034 1 Kit Ask for price

Human Cancer OptiTDS12: Tissue Dissociation System

4-28035 1 Kit Ask for price

Human Cancer OptiTDS13: Tissue Dissociation System

4-28036 1 Kit Ask for price

Human Cancer OptiTDS14: Tissue Dissociation System

4-28037 1 Kit Ask for price

Human Cancer OptiTDS15: Tissue Dissociation System

4-28038 1 Kit Ask for price

Human Cancer OptiTDS16: Tissue Dissociation System

4-28039 1 Kit Ask for price

Human Cancer OptiTDS18: Tissue Dissociation System

4-28041 1 Kit Ask for price

Human Cancer OptiTDS19: Tissue Dissociation System

4-28042 1 Kit Ask for price

Turbidity Std Ratio 4 NTU

CRSR-4-500 500ML
EUR 181.26

ICP-MS Tuning Solution 4

CL-TUNE-4 125ML
EUR 126.54

Adrenal Dissociation System 4 (Heart, Adrenal chromaffin, Paraneurons), Mouse and Rat

4-20204 ea Ask for price

Kidney Dissociation System 4 (Inner medullary duct,Papillae), Mouse and Rat

4-29294 ea Ask for price

Cancer Antigen CA 19-9 Antibody

abx023798-1mg 1 mg
EUR 1053.6

Tumor Dissociation System 4 (Epithelial tumor,Neoplastic, Tumor, islet), Mouse and Rat

4-20484 ea Ask for price

Turbidity Std Non Ratio 4 NTU

CRS-4-100 100ML
EUR 84.36

Turbidity Std Non Ratio 4 NTU

CRS-4-500 500ML
EUR 336.3

Human Colon Cancer Specific Antigen 4 (CCSA-4) ELISA Kit

abx354340-96tests 96 tests
EUR 848.4

Chicken Colon Cancer Specific Antigen-4 (CCSA-4) ELISA Kit

abx355731-96tests 96 tests
EUR 990

Rabbit Colon Cancer Specific Antigen-4 (CCSA-4) ELISA Kit

abx363654-96tests 96 tests
EUR 990

Monkey Colon Cancer Specific Antigen-4 (CCSA-4) ELISA Kit

abx358784-96tests 96 tests
EUR 990

Pig Colon Cancer Specific Antigen-4 (CCSA-4) ELISA Kit

abx360753-96tests 96 tests
EUR 990

Human colon cancer-specific antigen-4,CCSA-4 ELISA kit

201-12-1665 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human colon cancer-specific antigen-4(CCSA-4)ELISA Kit

GA-E1681HM-48T 48T
EUR 346.8

Human colon cancer-specific antigen-4(CCSA-4)ELISA Kit

GA-E1681HM-96T 96T
EUR 559.2

Human colon cancer-specific antigen-4(CCSA-4)ELISA Kit

QY-E03945 96T
EUR 433.2

Human colon cancer-specific antigen-4,CCSA-4 ELISA kit

YLA0017HU-48T 48T
EUR 435

Human colon cancer-specific antigen-4,CCSA-4 ELISA kit

YLA0017HU-96T 96T
EUR 562.5

Bovine Heat Shock Protein 72(HSP-72)

QY-E60086 96T
EUR 511.2

Paraffin Tissue Section - Human Bone Tumor: Chondrosarcoma, Grade I - II

T2235023-4 5 slides
EUR 308.4
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Quantifoil R 2/4

M-CEM-Q2100AR-4 100 GRIDS
EUR 895
Description: Quantifoil R 2/4; Gold 200 Mesh 100/pk

Quantifoil R 2/4

M-CEM-Q2100CR-4 100 GRIDS
EUR 700
Description: Quantifoil R 2/4; Copper 200 Mesh 100/pk

Quantifoil R 2/4

M-CEM-Q2100NR-4 100 GRIDS
EUR 800
Description: Quantifoil R 2/4; Nickel 200 Mesh 100/pk

In this study, we assigned each 3 clinical plasma samples into 6 aliquots to assess five EV commercial isolation kit, compared with ultracentrifugation (UC). We apply the standard EV preparation and transcriptome analysis workflow except EV isolation methods are used. Metrics EVs and RNA cargo (evRNA) compared to assess the technical variation versus biological variation in the clinical setting.