Design and Simulation of an Integrated Centrifugal Microfluidic Device for CTCs Separation and Cell Lysis
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Design and Simulation of an Integrated Centrifugal Microfluidic Device for CTCs Separation and Cell Lysis
Separation of circulating tumor cells (CTCs) from a blood sample and subsequent extraction of DNA from these cells play an important role in cancer research and drug discovery. Microfluidics is a versatile technology that has been applied to creating niche solutions for biomedical applications, such as cell separation and mixing, droplet generation, bioprinting, and organs on a chip. Centrifugal microfluidic biochips made on a compact disc showing great potential in processing biological samples for point of care diagnostics.
This study investigates the design and numerical simulation of integrated microfluidic devices, including cell separation unit to isolate CTCs from blood samples and micromixer unit for cell lysis on a rotating disk platform. For this purpose, the inertial microfluidic device designed for the separation of target cells using microchannel arrays contraction-expansion. In addition, the micromixer established to separate the target cells with a mixture of chemical reactants that dissolve the cell membrane lysis them to facilitate further tests. Our numerical simulations validated approach to both cell division and unit micromixer and strengthen the existing experimental results.
In the first compartment of the proposed device (cell separation unit), several simulations were carried out at a different angular velocity of 500 rpm to 3000 rpm to find the optimal angle speed for maximum separation efficiency. By using inertial separation of the proposed approach, CTCs, successfully separated from white blood cells (leukocytes) with a high efficiency (~ 90%) in the angular speed of 2000 rpm. In addition, the meandering channel with square obstacles designed to achieve highly efficient micromixer units with high quality mixing (~ 98%) to isolate CTCs lysis at 2000 rpm.
Design and Simulation of an Integrated Centrifugal Microfluidic Device for CTCs Separation and Cell Lysis
BIOCHEMICAL PROPERTIES OF THE NETWORK OF carboxypeptidase a UNTRANSFERRED AND FROM malignant neoplasms of the mammary gland
To study the biochemical properties of carboxypeptidase A from untransformed tissue and malignant neoplasms glands of mammals. Sampling anatomical materials for research conducted with adherence to ethical and legal standards.
Excretion of these enzymes include gradual fractionation with (NH4) 2SO4, dialysis in the presence of 2.0 мМ Zn ++ and gel chromatography on sephadex – G 75. Investigations substrate specificity of the enzyme substrate is held by hydrolysis carbobenzoxyphenylalanine, phenylalanylalanine, glutamyltyrosine, prolylalanine (2, 0 mM), hemoglobin and casein (2.0%). Effect of inhibitors and activators is determined in the presence of: Zn ++, cysteine, triton X-100, soybean trypsin inhibitor, leupeptin, pepstatin, PHMB, PMSF, dimethylmolyemydanhydride, tozylheptanol, mercaptoethanol, EDTA and 1.10 – fenantrolin.
Maximum velocity (Vmax), Mihaelis constant (Km), the type of inhibition and inhibition constant (Ki) were analyzed by Lineweaver – Burk method. Carboxypeptidase A from untransformed tissue and malignant neoplasms mammalian gland better divide the substrate, which has both hydrophobic and aromatic amino acids. A carboxypeptidase activity of mammalian gland malignant tumors was inhibited most of all under the influence of soybean trypsin inhibitor and PMSF, in contrast to untransformed tissue.
Description: A sandwich quantitative ELISA assay kit for detection of Human Carbohydrate Antigen 50 (CA50) in samples from serum, plasma or other biological fluids.
Description: A competitive ELISA for quantitative measurement of Human carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Quantitative sandwich ELISA kit for measuring Human carbohydrate antigen 50 (CA50) in samples from serum, plasma, cell culture supernates, asciticfluid, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative sandwich ELISA kit for measuring Human carbohydrate antigen 50(CA50) in samples from serum, plasma, cell culture supernates, asciticfluid, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: For the quantitative determination of human carbohydrate antigen 50(CA50) concentrations in serum, plasma, cell culture supernates, ascitic fluid, tissue homogenates, cell lysates.
Description: A competitive ELISA for quantitative measurement of Porcine carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Carbohydrate Antigen 50 (CA50) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Carbohydrate Antigen 50 (CA50) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Carbohydrate Antigen 50 (CA50) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Carbohydrate Antigen 50 (CA50) in serum, plasma and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Carbohydrate Antigen 50 (CA50) in samples from Serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Cancer Antigen 72-4 Antigen, Host/Source: Metastatic Liver. The purity is ~65% by SDS-PAGE. It is tested negative for HBsAg, antibodies to HCV and HIV 1/2.
Description: A competitive ELISA for quantitative measurement of Guinea pig carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig carbohyde antigen 50(CA50) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
ELISA kit for Human CA50 (Carbohydrate Antigen 50)
Description: A sandwich ELISA kit for detection of Carbohydrate Antigen 50 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
ELISA kit for Human carbohydrate antigen 50 (CA50)
Description: Enzyme-linked immunosorbent assay kit for quantification of Human carbohydrate antigen 50 (CA50) in samples from serum, plasma, tissue homogenates and other biological fluids.
ELISA kit for Human Carbohydrate antigen 50 (CA50)
Description: Quantitative sandwich ELISA for measuring Human Carbohydrate antigen 50 (CA50) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Carbohydrate antigen 50 (CA50)
Description: Quantitative sandwich ELISA for measuring Human Carbohydrate antigen 50 (CA50) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Carbohydrate antigen 50 (CA50)
Description: Quantitative sandwich ELISA for measuring Human Carbohydrate antigen 50 (CA50) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Cancer Antigen 19-9 Antigen, Host/Source: Liver Carcinoma. The purity is detected by gel filtration and ion-exchange chromatography. It is tested negative for HBsAg, antibodies to HCV and HIV 1/2.
To Carboxypeptidase A from untransformed tissue of the mammalian gland Km = 0.24 mM, and Ki = 0.40 mM determined, to carboxypeptidase A of a mammalian gland malignant neoplasms – Km = 0.17 mM, and Ki = 0, 65 mM. Carboxypeptidase A from untransformed tissue and mammalian gland malignant neoplasm identical to the specificity of the substrate
