Personalized medicine in oncology needs immunological test standards. Flow cytometry (FCM) method is an important tool for immunomonitoring, and their harmonization is essential to obtain comparable data in multicenter clinical trials. The purpose of this study was to design a workflow harmonization can address the problems most effectively contribute to intra- and inter-operator variability in project.
The multicenter Italian National Institute of Health (Istituto Superiore di Sanità, ISS) succeeded in multiparametric flow cytometric panel harmonization between thirteen owned operator of five clinical centers and research areas Lazio (Italy). panel based on a mixture backbone of antibodies dried (anti-CD3, anti-CD4, anti-CD8, anti-CD45RA and anti-CCR7) for detecting cell / memory T naive, is recognized as a potential prognostic / predictive immunological biomarkers in immunotherapies of cancer.
The coordination center congealed distributed peripheral blood mononuclear cells (PBMC) and a fresh sample of whole blood (WB) from a healthy donor, reagents and standard operating procedures (SOP) for participants to experiment with their own equipment, to simulate a real-life scenario. Operators and local back raw data were analyzed for the ISS for central analysis and statistics elaboration.Harmonised and reproducible results are obtained by sharing the experimental set-up and procedures along with the centralization of data analysis, which leads to a reduction in cross-center variability naive / memory section in particular frequency whole blood setting.
Our experimental and analytical work processes proved to be suitable for harmonization of FCM test in a multicenter setting, where high quality data needed to evaluate the potential for immunological markers, which can contribute to choose a better therapeutic selection.
Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naïve/Memory T Cell Immunomonitoring.
RNAseq in comparison to Immunohistochemistry to Measure Cancer Biomarkers in Breast Cancer and Lung Cancer Specimens.
RNA sequencing is considered the gold standard for high-throughput gene expression profile at the level of transcription. increasingly important in cancer research and molecular diagnostics is reflected in the growing number of mentions in the scientific literature and clinical trials reports. However, the use of different reagents and protocols for RNA sequencing often produce results that are not compatible.
More recently, we published a profile of Atlas Oncobox RNA sequencing to normal human tissues were obtained from a healthy donor were killed in road accidents. This is a database of molecular profiles obtained using the setting uniform protocol and reagents that can be widely used in biomedicine for the normalization of data in pathology, including cancer.
Here, we publish 39 original new breast cancer (BC) and the profile 19 of lung cancer (LC) RNA sequencing was obtained for paraffin-embedded samples (FFPE) tissue formalin-fixed, fully compatible with Oncobox Atlas. We did the first correlation study RNA sequencing and immunohistochemical expression profile-measured for actionable clinical biomarker genes in the cancer FFPE tissue samples. We showed a high (Spearman’s rho 0.65 to 0.798) and statistically significant (p <0.00004) correlation between RNA sequencing (Oncobox protocol) and immunohistochemical measurement of HER2 / ERBB2, ER / ESR1 and PGR gene in BC, and for gene PDL1 The LC; AUC: 0.963 to HER2, 0.921 for ESR1, 0.912 to PGR, and 0.922 for PDL1.
Cancer Antigen 125 (CA 125), Antibody
GWB-10B890
GenWay Biotech
1 mg
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Cancer Antigen 125 (CA 125), Antibody
GWB-85FE2C
GenWay Biotech
1 mg
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Cancer Antigen 125 (CA 125), Antibody
GWB-9D1541
GenWay Biotech
1 mg
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Cancer Antigen 125 (CA 125), Antibody
GWB-A64DAC
GenWay Biotech
1 mg
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Cancer Antigen 125 (CA 125), Antibody
GWB-6D5E80
GenWay Biotech
1 mg
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Cancer Antigen 125 (CA 125), Antibody
GWB-F984F6
GenWay Biotech
1 mg
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Cancer Antigen 125 (CA 125) -HRP, Antibody
GWB-D528AD
GenWay Biotech
0.5 mg
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Cancer Antigen 125 (CA-125), Human
MBS173332-100KUnits
MyBiosource
100KUnits
EUR 1595
Cancer Antigen 125 (CA-125), Human
MBS173332-50KUnits
MyBiosource
50KUnits
EUR 930
Cancer Antigen 125 (CA-125), Human
MBS173332-5x100KUnits
MyBiosource
5x100KUnits
EUR 6965
Cancer Antigen 125 (CA 125) High Purity
GWB-B446E0
GenWay Biotech
50000 Unit
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Cancer Antigen 125 (CA 125) Standard Grade
GWB-565817
GenWay Biotech
25000 Unit
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Cancer Antigen 125 (CA 125) Standard Grade
GWB-A9985A
GenWay Biotech
50000 Unit
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CA 125 (Ovarian Cancer Antigen) Antibody, Concentrate
MAB559C
Innovex
0.2ml
EUR 455
Cancer Antigen 125 (CA-125) Mouse Monoclonal anti-Human Antibody
MBS170048-01mg
MyBiosource
0.1mg
EUR 315
Cancer Antigen 125 (CA-125) Mouse Monoclonal anti-Human Antibody
MBS170048-1mg
MyBiosource
1mg
EUR 735
Cancer Antigen 125 (CA-125) Mouse Monoclonal anti-Human Antibody
MBS170048-5x1mg
MyBiosource
5x1mg
EUR 3105
Cancer Antigen 125 (CA-125) Mouse Monoclonal anti-Human Antibody
MBS170068-01mg
MyBiosource
0.1mg
EUR 315
Cancer Antigen 125 (CA-125) Mouse Monoclonal anti-Human Antibody
MBS170068-5x01mg
MyBiosource
5x0.1mg
EUR 1205
Cancer Antigen 125 (CA 125) Low Cross Grade
GWB-87F2DB
GenWay Biotech
10000 Unit
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Human Cancer Antigen 125 (CA 125) ELISA Kit
PRB-5061
Cell Biolabs
96 assays
EUR 686.4
Human Cancer Antigen 125 (CA 125) ELISA Kit
PRB-5061-5
Cell Biolabs
5 x 96 assays
EUR 2739.6
Cancer Antigen 125 (CA-125) (human) ELISA Kit
K4803-100
Biovision
each
EUR 1038
Cancer Antigen 125 (CA125) Antibody-BIOTIN
E63C01603
EnoGene
100μg/100μl
EUR 275
Description: Available in various conjugation types.
Cancer Antigen 125 (CA125) Antibody-BIOTIN
MBS850751-01mg
MyBiosource
0.1mg
EUR 345
Cancer Antigen 125 (CA125) Antibody-BIOTIN
MBS850751-5x01mg
MyBiosource
5x0.1mg
EUR 1410
Cancer Antigen CA-125 Antigen (Human Fluids)
VAng-Wyb8639-inquire
Creative Biolabs
inquire
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Description: CA-125, Calibrator Grade, cancer antigen from Human Fluids, 53.26 KU/mL.
Cancer Antigen CA-125 Antigen (Human Adenocarcinoma)
VAng-Wyb8642-inquire
Creative Biolabs
inquire
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Description: CA-125, Calibrator Grade, Cancer antigen from Human Adenocarcinoma, 500 kIU / mL.
Cancer Antigen 125 (CA125) Antibody
E63C01601
EnoGene
100μg/100μl
EUR 225
Description: Available in various conjugation types.
Cancer Antigen 125 (CA125) Antibody
MBS850553-01mg
MyBiosource
0.1mg
EUR 305
Cancer Antigen 125 (CA125) Antibody
MBS850553-01mLAF405L
MyBiosource
0.1mL(AF405L)
EUR 465
Cancer Antigen 125 (CA125) Antibody
MBS850553-01mLAF405S
MyBiosource
0.1mL(AF405S)
EUR 465
Cancer Antigen 125 (CA125) Antibody
MBS850553-01mLAF610
MyBiosource
0.1mL(AF610)
EUR 465
Cancer Antigen 125 (CA125) Antibody
MBS850553-01mLAF635
MyBiosource
0.1mL(AF635)
EUR 465
Cancer Antigen 125 (CA 125) High Purity Low Cross Grade
GWB-12E060
GenWay Biotech
20000 Unit
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Cancer Antigen 125 (CA 125) Cell Culture Low Cross Grade
GWB-143910
GenWay Biotech
25000 Unit
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CA 125 (Ovarian Cancer Antigen) Antibody, Ready-To-Use
MAB559P
Innovex
5ml
EUR 435
Cancer Antigent (CA) 125 ELISA
GWB-BQK04B
GenWay Biotech
NA
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Cancer Antigen 125 (CA125) Antibody-HRP
E63C01602
EnoGene
100μg/100μl
EUR 275
Description: Available in various conjugation types.
Cancer Antigen 125 (CA125) Antibody-HRP
MBS850442-01mg
MyBiosource
0.1mg
EUR 345
Cancer Antigen 125 (CA125) Antibody-HRP
MBS850442-5x01mg
MyBiosource
5x0.1mg
EUR 1410
Cancer Antigen CA-125 Antigen (Human Fluids) (Partially Pure)
VAng-Wyb8637-inquire
Creative Biolabs
inquire
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Description: CA-125 (Ovarian Cancer), Partially Pure, Cancer antigen from Human Fluids, 73.90 kUnits/mL.
Cancer Antigen CA-125 Antigen (Ovarian Carcinoma Cell Line)
VAng-Wyb8641-inquire
Creative Biolabs
inquire
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Description: CA-125, Antigen Grade, Cancer antigen from Ovarian Carcinoma Cell Line, 84,040 Units/mL.
Mouse Monoclonal anti-human Cancer Antigen (CA)-125
hAP-1124
Angio Proteomie
50ug
EUR 400
Mouse Monoclonal anti-human Cancer Antigen (CA)-125
hAP-1124A
Angio Proteomie
50ug
EUR 400
Mucin 16 (MUC16, MUC-16, CA125, CA-125, Cancer Antigen 125, FLJ14303, Mucin 16 Cell Surface Associated, Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125) (Biotin)
MBS6249182-01mL
MyBiosource
0.1(mL
EUR 995
Mucin 16 (MUC16, MUC-16, CA125, CA-125, Cancer Antigen 125, FLJ14303, Mucin 16 Cell Surface Associated, Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125) (Biotin)
MBS6249182-5x01mL
MyBiosource
5x0.1mL
EUR 4325
Mucin 16 (MUC16, MUC-16, CA125, CA-125, Cancer Antigen 125, FLJ14303, Mucin 16 Cell Surface Associated, Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125) (Biotin)
MBS6249183-01mL
MyBiosource
0.1(mL
EUR 870
Mucin 16 (MUC16, MUC-16, CA125, CA-125, Cancer Antigen 125, FLJ14303, Mucin 16 Cell Surface Associated, Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125) (Biotin)
MBS6249183-5x01mL
MyBiosource
5x0.1mL
EUR 3765
Cancer Antigen 125 Antigen (Ascites Fluid)
VAng-Cr3870-10kU
Creative Biolabs
10 kU
EUR 1132.8
Description: Cancer Antigen 125 Antigen, Host/Source: Ascites Fluid. The purity is ≥ 95% by SDS-PAGE.
Human Mucin-16 (MUC-16, CA-125) Antibody (Biotin Conjugate)
32189-05121
AssayPro
150 ug
EUR 315
Cancer Antigen 125 Antigen (Human Adenocarcinoma)
VAng-Cr3868-10kU
Creative Biolabs
10 kU
EUR 505.2
Description: Cancer Antigen 125 Antigen, Host/Source: Human Adenocarcinoma. The purity is detected by salt extraction and chromatography.
Cancer Antigen 125 Antigen (Human Adenocarcinoma) (> 90%)
VAng-Cr3869-25kU
Creative Biolabs
25 kU
EUR 967.2
Description: Cancer Antigen 125 Antigen, Host/Source: Human Adenocarcinoma. The purity is> 90% by SDS-PAGE. Matched Pair: HM183, HM184, HM185, HM186.
Human Cancer Antigen 125 (CA125) Protein
abx061598-250kU
Abbexa
250 kU
EUR 1195.2
Human Cancer Antigen 125 (CA125) Protein
abx061598-1mg
Abbexa
1 mg
EUR 1062.5
Human Cancer Antigen 125 (CA125) Protein
abx061598-5mg
Abbexa
5 mg
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Rabbit anti-human Breast cancer type 1 susceptibility protein polyclonal Antibody, Biotin conjugated
MBS719718-005mg
MyBiosource
0.05mg
EUR 190
Rabbit anti-human Breast cancer type 1 susceptibility protein polyclonal Antibody, Biotin conjugated
MBS719718-01mg
MyBiosource
0.1mg
EUR 270
Rabbit anti-human Breast cancer type 1 susceptibility protein polyclonal Antibody, Biotin conjugated
MBS719718-5x01mg
MyBiosource
5x0.1mg
EUR 1205
CA 125 antibody (biotin)
61R-C112bBT
Fitzgerald
1 mg
EUR 406
Description: Mouse monoclonal CA 125 antibody (biotin)
CA 125 antibody (biotin)
MBS531893-1mg
MyBiosource
1mg
EUR 545
CA 125 antibody (biotin)
MBS531893-5x1mg
MyBiosource
5x1mg
EUR 2295
CA125 Cancer Antigen (Ovarian Cancer) (Biotin)
MBS6261311-01mL
MyBiosource
0.1mL
EUR 970
CA125 Cancer Antigen (Ovarian Cancer) (Biotin)
MBS6261311-5x01mL
MyBiosource
5x0.1mL
EUR 4220
Rabbit anti-human Vesicular, overexpressed in cancer, prosurvival protein 1 polyclonal Antibody, Biotin conjugated
MBS715766-005mg
MyBiosource
0.05mg
EUR 190
Rabbit anti-human Vesicular, overexpressed in cancer, prosurvival protein 1 polyclonal Antibody, Biotin conjugated
MBS715766-01mg
MyBiosource
0.1mg
EUR 270
Rabbit anti-human Vesicular, overexpressed in cancer, prosurvival protein 1 polyclonal Antibody, Biotin conjugated
MBS715766-5x01mg
MyBiosource
5x0.1mg
EUR 1205
Rabbit anti-human Vesicular, overexpressed in cancer, prosurvival protein 1 polyclonal Antibody, Biotin conjugated
MBS716030-005mg
MyBiosource
0.05mg
EUR 190
Rabbit anti-human Vesicular, overexpressed in cancer, prosurvival protein 1 polyclonal Antibody, Biotin conjugated
MBS716030-01mg
MyBiosource
0.1mg
EUR 270
Rabbit anti-human Vesicular, overexpressed in cancer, prosurvival protein 1 polyclonal Antibody, Biotin conjugated
MBS716030-5x01mg
MyBiosource
5x0.1mg
EUR 1205
Cancer/Testis Antigen 5.1 (SSX1) Antibody (Biotin)
20-abx316208
Abbexa
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100 ug
1 mg
200 ug
20 ug
50 ug
Cancer/Testis Antigen 5.1 (SSX1) Antibody (Biotin)
abx316208-100g
Abbexa
100 µg
EUR 362.5
Cancer/Testis Antigen 5.1 (SSX1) Antibody (Biotin)
abx316208-20g
Abbexa
20 µg
EUR 162.5
Cancer/Testis Antigen 5.1 (SSX1) Antibody (Biotin)
abx316208-50g
Abbexa
50 µg
EUR 250
Human Sialylated Lewis A Antigen (CA 19-9) Antibody (Biotin Conjugate)
30261-05121
AssayPro
150 ug
EUR 315
OASA08742-1ML - Salmonella Group Antigen Antibody - Biotin Conjugated
OASA08742-1ML
Aviva Systems Biology
1ml
EUR 479
Cancer Antigen CA19-9 (Gastrointestinal Cancer) (Biotin)
MBS6127297-01mL
MyBiosource
0.1(mL
EUR 1060
Cancer Antigen CA19-9 (Gastrointestinal Cancer) (Biotin)
MBS6127297-5x01mL
MyBiosource
5x0.1mL
EUR 4615
Cancer Antigen CA19-9 (Gastrointestinal Cancer) (Biotin)
MBS6127298-01mL
MyBiosource
0.1(mL
EUR 1060
Cancer Antigen CA19-9 (Gastrointestinal Cancer) (Biotin)
MBS6127298-5x01mL
MyBiosource
5x0.1mL
EUR 4615
Cancer Antigen CA19-9 (Gastrointestinal Cancer) (Biotin)
MBS6127299-01mL
MyBiosource
0.1(mL
EUR 1060
Cancer Antigen CA19-9 (Gastrointestinal Cancer) (Biotin)
MBS6127299-5x01mL
MyBiosource
5x0.1mL
EUR 4615
Rabbit anti-Mouse CD63 antigen polyclonal Antibody, Biotin conjugated
MBS1488249-005mg
MyBiosource
0.05mg
EUR 190
Rabbit anti-Mouse CD63 antigen polyclonal Antibody, Biotin conjugated
MBS1488249-01mg
MyBiosource
0.1mg
EUR 270
Rabbit anti-Mouse CD63 antigen polyclonal Antibody, Biotin conjugated
MBS1488249-5x01mg
MyBiosource
5x0.1mg
EUR 1205
Rabbit anti-mouse CD82 antigen polyclonal Antibody, Biotin conjugated
MBS1491466-005mg
MyBiosource
0.05mg
EUR 190
Rabbit anti-mouse CD82 antigen polyclonal Antibody, Biotin conjugated
MBS1491466-01mg
MyBiosource
0.1mg
EUR 270
Rabbit anti-mouse CD82 antigen polyclonal Antibody, Biotin conjugated
MBS1491466-5x01mg
MyBiosource
5x0.1mg
EUR 1205
CA19-9 (Cancer, Cancer antigen 19-9, CA199) (Biotin)
MBS6261468-01mL
MyBiosource
0.1mL
EUR 1085
CA19-9 (Cancer, Cancer antigen 19-9, CA199) (Biotin)
MBS6261468-5x01mL
MyBiosource
5x0.1mL
EUR 4725
CA125 Cancer Antigen, A Domain (Ovarian Cancer) (Biotin)
MBS6127289-01mL
MyBiosource
0.1(mL
EUR 1350
CA125 Cancer Antigen, A Domain (Ovarian Cancer) (Biotin)
MBS6127289-5x01mL
MyBiosource
5x0.1mL
EUR 5920
CA125 Cancer Antigen, B Domain (Ovarian Cancer) (Biotin)
MBS6127290-01mL
MyBiosource
0.1(mL
EUR 1350
CA125 Cancer Antigen, B Domain (Ovarian Cancer) (Biotin)
MBS6127290-5x01mL
MyBiosource
5x0.1mL
EUR 5920
Carbohydrate Antigen 125 (CA125) Antibody (Biotin)
20-abx272665
Abbexa
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100 ug
10 ug
1 mg
200 ug
50 ug
Carbohydrate Antigen 125 (CA125) Antibody (Biotin)
20-abx271489
Abbexa
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100 ug
10 ug
1 mg
200 ug
50 ug
Carbohydrate Antigen 125 (CA125) Antibody (Biotin)
abx271489-1ml
Abbexa
1 ml
EUR 737.5
Carbohydrate Antigen 125 (CA125) Antibody (Biotin)
abx271489-200l
Abbexa
200 µl
EUR 337.5
Carbohydrate Antigen 125 (CA125) Antibody (Biotin)
abx272665-100l
Abbexa
100 µl
EUR 337.5
Carbohydrate Antigen 125 (CA125) Antibody (Biotin)
abx272665-1ml
Abbexa
1 ml
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Carbohydrate Antigen 125 (CA125) Antibody (Biotin)
abx272665-200l
Abbexa
200 µl
EUR 762.5
Rabbit anti-human G antigen 5 polyclonal Antibody, Biotin conjugated
MBS1488890-005mg
MyBiosource
0.05mg
EUR 190
Rabbit anti-human G antigen 5 polyclonal Antibody, Biotin conjugated
MBS1488890-01mg
MyBiosource
0.1mg
EUR 270
Rabbit anti-human G antigen 5 polyclonal Antibody, Biotin conjugated
MBS1488890-5x01mg
MyBiosource
5x0.1mg
EUR 1205
Cancer Antigen CA 19-9 Antibody
abx023798-1mg
Abbexa
1 mg
EUR 1053.6
Rabbit anti-human Antigen KI-67 polyclonal Antibody, Biotin conjugated
MBS1488970-005mg
MyBiosource
0.05mg
EUR 190
Rabbit anti-human Antigen KI-67 polyclonal Antibody, Biotin conjugated
MBS1488970-01mg
MyBiosource
0.1mg
EUR 270
Rabbit anti-human Antigen KI-67 polyclonal Antibody, Biotin conjugated
MBS1488970-5x01mg
MyBiosource
5x0.1mg
EUR 1205
CA125 Cancer Antigen (Ovarian Cancer) (Glycerol Free) (Biotin)
MBS6261276-01mL
MyBiosource
0.1mL
EUR 970
CA125 Cancer Antigen (Ovarian Cancer) (Glycerol Free) (Biotin)
MBS6261276-5x01mL
MyBiosource
5x0.1mL
EUR 4220
Cancer Antigen 50 (CA 50), Antibody
GWB-737484
GenWay Biotech
1 mg
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MUC16 (Mucin-16, MUC-16, CA-125, Ovarian Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125, CA125, FLJ14303) (Biotin)
MBS6261406-01mL
MyBiosource
0.1mL
EUR 970
MUC16 (Mucin-16, MUC-16, CA-125, Ovarian Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125, CA125, FLJ14303) (Biotin)
MBS6261406-5x01mL
MyBiosource
5x0.1mL
EUR 4220
MUC16 (Mucin-16, MUC-16, CA-125, Ovarian Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125, CA125, FLJ14303) (Biotin)
MBS6261407-01mL
MyBiosource
0.1mL
EUR 970
MUC16 (Mucin-16, MUC-16, CA-125, Ovarian Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125, CA125, FLJ14303) (Biotin)
MBS6261407-5x01mL
MyBiosource
5x0.1mL
EUR 4220
MUC16 (Mucin-16, MUC-16, CA-125, Ovarian Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125, CA125, FLJ14303) (Biotin)
MBS6261408-01mL
MyBiosource
0.1mL
EUR 920
MUC16 (Mucin-16, MUC-16, CA-125, Ovarian Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125, CA125, FLJ14303) (Biotin)
MBS6261408-5x01mL
MyBiosource
5x0.1mL
EUR 3980
MUC16 (Mucin-16, MUC-16, CA-125, Ovarian Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125, CA125, FLJ14303) (Biotin)
MBS6261409-01mL
MyBiosource
0.1mL
EUR 1095
MUC16 (Mucin-16, MUC-16, CA-125, Ovarian Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125, CA125, FLJ14303) (Biotin)
MBS6261409-5x01mL
MyBiosource
5x0.1mL
EUR 4785
Rabbit anti-Toxocara canis 26 kDa secreted antigen polyclonal Antibody, Biotin conjugated
MBS1493177-005mg
MyBiosource
0.05mg
EUR 190
Rabbit anti-Toxocara canis 26 kDa secreted antigen polyclonal Antibody, Biotin conjugated
MBS1493177-01mg
MyBiosource
0.1mg
EUR 270
To our knowledge, this is the first validation total RNA sequencing archived FFPE material provide a reliable estimate of the level of the marker protein. These results suggest that in the future, RNA sequencing can equip immunohistochemistry for the reliable measurement of biomarker expression in FFPE cancer samples.