Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naïve/Memory T Cell Immunomonitoring.

Personalized medicine in oncology needs immunological test standards. Flow cytometry (FCM) method is an important tool for immunomonitoring, and their harmonization is essential to obtain comparable data in multicenter clinical trials. The purpose of this study was to design a workflow harmonization can address the problems most effectively contribute to intra- and inter-operator variability in project.

The multicenter Italian National Institute of Health (Istituto Superiore di Sanità, ISS) succeeded in multiparametric flow cytometric panel harmonization between thirteen owned operator of five clinical centers and research areas Lazio (Italy). panel based on a mixture backbone of antibodies dried (anti-CD3, anti-CD4, anti-CD8, anti-CD45RA and anti-CCR7) for detecting cell / memory T naive, is recognized as a potential prognostic / predictive immunological biomarkers in immunotherapies of cancer.

The coordination center congealed distributed peripheral blood mononuclear cells (PBMC) and a fresh sample of whole blood (WB) from a healthy donor, reagents and standard operating procedures (SOP) for participants to experiment with their own equipment, to simulate a real-life scenario. Operators and local back raw data were analyzed for the ISS for central analysis and statistics elaboration.Harmonised and reproducible results are obtained by sharing the experimental set-up and procedures along with the centralization of data analysis, which leads to a reduction in cross-center variability naive / memory section in particular frequency whole blood setting.

Our experimental and analytical work processes proved to be suitable for harmonization of FCM test in a multicenter setting, where high quality data needed to evaluate the potential for immunological markers, which can contribute to choose a better therapeutic selection.

 Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naïve/Memory T Cell Immunomonitoring.
Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naïve/Memory T Cell Immunomonitoring.

RNAseq in comparison to Immunohistochemistry to Measure Cancer Biomarkers in Breast Cancer and Lung Cancer Specimens.

RNA sequencing is considered the gold standard for high-throughput gene expression profile at the level of transcription. increasingly important in cancer research and molecular diagnostics is reflected in the growing number of mentions in the scientific literature and clinical trials reports. However, the use of different reagents and protocols for RNA sequencing often produce results that are not compatible.

More recently, we published a profile of Atlas Oncobox RNA sequencing to normal human tissues were obtained from a healthy donor were killed in road accidents. This is a database of molecular profiles obtained using the setting uniform protocol and reagents that can be widely used in biomedicine for the normalization of data in pathology, including cancer.

Here, we publish 39 original new breast cancer (BC) and the profile 19 of lung cancer (LC) RNA sequencing was obtained for paraffin-embedded samples (FFPE) tissue formalin-fixed, fully compatible with Oncobox Atlas. We did the first correlation study RNA sequencing and immunohistochemical expression profile-measured for actionable clinical biomarker genes in the cancer FFPE tissue samples. We showed a high (Spearman’s rho 0.65 to 0.798) and statistically significant (p <0.00004) correlation between RNA sequencing (Oncobox protocol) and immunohistochemical measurement of HER2 / ERBB2, ER / ESR1 and PGR gene in BC, and for gene PDL1 The LC; AUC: 0.963 to HER2, 0.921 for ESR1, 0.912 to PGR, and 0.922 for PDL1.

Cancer Antigen 125 (CA 125), Antibody

GWB-10B890 1 mg Ask for price

Cancer Antigen 125 (CA 125), Antibody

GWB-85FE2C 1 mg Ask for price

Cancer Antigen 125 (CA 125), Antibody

GWB-9D1541 1 mg Ask for price

Cancer Antigen 125 (CA 125), Antibody

GWB-A64DAC 1 mg Ask for price

Cancer Antigen 125 (CA 125), Antibody

GWB-6D5E80 1 mg Ask for price

Cancer Antigen 125 (CA 125), Antibody

GWB-F984F6 1 mg Ask for price

Cancer Antigen 125 (CA 125) -HRP, Antibody

GWB-D528AD 0.5 mg Ask for price

OAEF00238-1MG - Cancer Antigen 125 (CA 125) Antibody

OAEF00238-1MG 1mg
EUR 279

OAEF00239-1MG - Cancer Antigen 125 (CA 125) Antibody

OAEF00239-1MG 1mg
EUR 279

OAEF00240-1MG - Cancer Antigen 125 (CA 125) Antibody

OAEF00240-1MG 1mg
EUR 279

OAEF00263-1MG - Cancer Antigen 125 (CA 125) Antibody

OAEF00263-1MG 1mg
EUR 279

OAEF00264-1MG - Cancer Antigen 125 (CA 125) Antibody

OAEF00264-1MG 1mg
EUR 279

OAEF00265-1MG - Cancer Antigen 125 (CA 125) Antibody

OAEF00265-1MG 1mg
EUR 279

OAEF00266-1MG - Cancer Antigen 125 (CA 125) Antibody

OAEF00266-1MG 1mg
EUR 279

Cancer Antigen 125 (CA-125), Human

MBS173332-100KUnits 100KUnits
EUR 1595

Cancer Antigen 125 (CA-125), Human

MBS173332-50KUnits 50KUnits
EUR 930

Cancer Antigen 125 (CA-125), Human

MBS173332-5x100KUnits 5x100KUnits
EUR 6965

OPEF01468-10KU - Cancer Antigen 125 (CA 125) Antigen

OPEF01468-10KU 10kU
EUR 185

OPEF01584-25KU - Cancer Antigen 125 (CA 125) Antigen

OPEF01584-25KU 25kU
EUR 377

Cancer Antigen 125 (CA 125) High Purity

GWB-B446E0 50000 Unit Ask for price

Cancer Antigen 125 (CA 125) Standard Grade

GWB-565817 25000 Unit Ask for price

Cancer Antigen 125 (CA 125) Standard Grade

GWB-A9985A 50000 Unit Ask for price

CA 125 (Ovarian Cancer Antigen) Antibody, Concentrate

MAB559C 0.2ml
EUR 455

Ovarian Cancer Antigen (CA 125)

CA1251-N-10 10 KU
EUR 634.8

Cancer Antigen 125 (CA-125) Mouse Monoclonal anti-Human Antibody

MBS170048-01mg 0.1mg
EUR 315

Cancer Antigen 125 (CA-125) Mouse Monoclonal anti-Human Antibody

MBS170048-1mg 1mg
EUR 735

Cancer Antigen 125 (CA-125) Mouse Monoclonal anti-Human Antibody

MBS170048-5x1mg 5x1mg
EUR 3105

Cancer Antigen 125 (CA-125) Mouse Monoclonal anti-Human Antibody

MBS170068-01mg 0.1mg
EUR 315

Cancer Antigen 125 (CA-125) Mouse Monoclonal anti-Human Antibody

MBS170068-5x01mg 5x0.1mg
EUR 1205

Cancer Antigen 125 (CA 125) Low Cross Grade

GWB-87F2DB 10000 Unit Ask for price

Human Cancer Antigen 125 (CA 125) ELISA Kit

PRB-5061 96 assays
EUR 686.4

Human Cancer Antigen 125 (CA 125) ELISA Kit

PRB-5061-5 5 x 96 assays
EUR 2739.6

Cancer Antigen 125 (CA-125) (human) ELISA Kit

K4803-100 each
EUR 1038

Cancer Antigen 125 (CA125) Antibody-BIOTIN

E63C01603 100μg/100μl
EUR 275
Description: Available in various conjugation types.

Cancer Antigen 125 (CA125) Antibody-BIOTIN

MBS850751-01mg 0.1mg
EUR 345

Cancer Antigen 125 (CA125) Antibody-BIOTIN

MBS850751-5x01mg 5x0.1mg
EUR 1410

Cancer Antigen CA-125 Antigen (Human Fluids)

VAng-Wyb8639-inquire inquire Ask for price
Description: CA-125, Calibrator Grade, cancer antigen from Human Fluids, 53.26 KU/mL.

Cancer Antigen CA-125 Antigen (Human Adenocarcinoma)

VAng-Wyb8642-inquire inquire Ask for price
Description: CA-125, Calibrator Grade, Cancer antigen from Human Adenocarcinoma, 500 kIU / mL.

Cancer Antigen 125 (CA125) Antibody

E63C01601 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Cancer Antigen 125 (CA125) Antibody

MBS850553-01mg 0.1mg
EUR 305

Cancer Antigen 125 (CA125) Antibody

MBS850553-01mLAF405L 0.1mL(AF405L)
EUR 465

Cancer Antigen 125 (CA125) Antibody

MBS850553-01mLAF405S 0.1mL(AF405S)
EUR 465

Cancer Antigen 125 (CA125) Antibody

MBS850553-01mLAF610 0.1mL(AF610)
EUR 465

Cancer Antigen 125 (CA125) Antibody

MBS850553-01mLAF635 0.1mL(AF635)
EUR 465

Cancer Antigen 125 (CA 125) High Purity Low Cross Grade

GWB-12E060 20000 Unit Ask for price

Cancer Antigen 125 (CA 125) Cell Culture Low Cross Grade

GWB-143910 25000 Unit Ask for price

CA 125 (Ovarian Cancer Antigen) Antibody, Ready-To-Use

MAB559P 5ml
EUR 435

Cancer Antigent (CA) 125 ELISA

GWB-BQK04B NA Ask for price

Cancer Antigen 125 (CA125) Antibody-HRP

E63C01602 100μg/100μl
EUR 275
Description: Available in various conjugation types.

Cancer Antigen 125 (CA125) Antibody-HRP

MBS850442-01mg 0.1mg
EUR 345

Cancer Antigen 125 (CA125) Antibody-HRP

MBS850442-5x01mg 5x0.1mg
EUR 1410

Cancer Antigen CA-125 Antigen (Human Fluids) (Partially Pure)

VAng-Wyb8637-inquire inquire Ask for price
Description: CA-125 (Ovarian Cancer), Partially Pure, Cancer antigen from Human Fluids, 73.90 kUnits/mL.

Cancer Antigen CA-125 Antigen (Ovarian Carcinoma Cell Line)

VAng-Wyb8641-inquire inquire Ask for price
Description: CA-125, Antigen Grade, Cancer antigen from Ovarian Carcinoma Cell Line, 84,040 Units/mL.

Mouse Monoclonal anti-human Cancer Antigen (CA)-125

hAP-1124 50ug
EUR 400

Mouse Monoclonal anti-human Cancer Antigen (CA)-125

hAP-1124A 50ug
EUR 400

Mucin 16 (MUC16, MUC-16, CA125, CA-125, Cancer Antigen 125, FLJ14303, Mucin 16 Cell Surface Associated, Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125) (Biotin)

MBS6249182-01mL 0.1(mL
EUR 995

Mucin 16 (MUC16, MUC-16, CA125, CA-125, Cancer Antigen 125, FLJ14303, Mucin 16 Cell Surface Associated, Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125) (Biotin)

MBS6249182-5x01mL 5x0.1mL
EUR 4325

Mucin 16 (MUC16, MUC-16, CA125, CA-125, Cancer Antigen 125, FLJ14303, Mucin 16 Cell Surface Associated, Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125) (Biotin)

MBS6249183-01mL 0.1(mL
EUR 870

Mucin 16 (MUC16, MUC-16, CA125, CA-125, Cancer Antigen 125, FLJ14303, Mucin 16 Cell Surface Associated, Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125) (Biotin)

MBS6249183-5x01mL 5x0.1mL
EUR 3765

Cancer Antigen 125 Antigen (Ascites Fluid)

VAng-Cr3870-10kU 10 kU
EUR 1132.8
Description: Cancer Antigen 125 Antigen, Host/Source: Ascites Fluid. The purity is ≥ 95% by SDS-PAGE.

Human Mucin-16 (MUC-16, CA-125) Antibody (Biotin Conjugate)

32189-05121 150 ug
EUR 315

Cancer Antigen 125 Antigen (Human Adenocarcinoma)

VAng-Cr3868-10kU 10 kU
EUR 505.2
Description: Cancer Antigen 125 Antigen, Host/Source: Human Adenocarcinoma. The purity is detected by salt extraction and chromatography.

Cancer Antigen 125 Antigen (Human Adenocarcinoma) (> 90%)

VAng-Cr3869-25kU 25 kU
EUR 967.2
Description: Cancer Antigen 125 Antigen, Host/Source: Human Adenocarcinoma. The purity is> 90% by SDS-PAGE. Matched Pair: HM183, HM184, HM185, HM186.

Human Cancer Antigen 125 (CA125) Protein

abx061598-250kU 250 kU
EUR 1195.2

Human Cancer Antigen 125 (CA125) Protein

abx061598-1mg 1 mg
EUR 1062.5

Human Cancer Antigen 125 (CA125) Protein

abx061598-5mg 5 mg Ask for price

Rabbit anti-human Breast cancer type 1 susceptibility protein polyclonal Antibody, Biotin conjugated

MBS719718-005mg 0.05mg
EUR 190

Rabbit anti-human Breast cancer type 1 susceptibility protein polyclonal Antibody, Biotin conjugated

MBS719718-01mg 0.1mg
EUR 270

Rabbit anti-human Breast cancer type 1 susceptibility protein polyclonal Antibody, Biotin conjugated

MBS719718-5x01mg 5x0.1mg
EUR 1205

CA 125 antibody (biotin)

61R-C112bBT 1 mg
EUR 406
Description: Mouse monoclonal CA 125 antibody (biotin)

CA 125 antibody (biotin)

MBS531893-1mg 1mg
EUR 545

CA 125 antibody (biotin)

MBS531893-5x1mg 5x1mg
EUR 2295

CA125 Cancer Antigen (Ovarian Cancer) (Biotin)

MBS6261311-01mL 0.1mL
EUR 970

CA125 Cancer Antigen (Ovarian Cancer) (Biotin)

MBS6261311-5x01mL 5x0.1mL
EUR 4220

Rabbit anti-human Vesicular, overexpressed in cancer, prosurvival protein 1 polyclonal Antibody, Biotin conjugated

MBS715766-005mg 0.05mg
EUR 190

Rabbit anti-human Vesicular, overexpressed in cancer, prosurvival protein 1 polyclonal Antibody, Biotin conjugated

MBS715766-01mg 0.1mg
EUR 270

Rabbit anti-human Vesicular, overexpressed in cancer, prosurvival protein 1 polyclonal Antibody, Biotin conjugated

MBS715766-5x01mg 5x0.1mg
EUR 1205

Rabbit anti-human Vesicular, overexpressed in cancer, prosurvival protein 1 polyclonal Antibody, Biotin conjugated

MBS716030-005mg 0.05mg
EUR 190

Rabbit anti-human Vesicular, overexpressed in cancer, prosurvival protein 1 polyclonal Antibody, Biotin conjugated

MBS716030-01mg 0.1mg
EUR 270

Rabbit anti-human Vesicular, overexpressed in cancer, prosurvival protein 1 polyclonal Antibody, Biotin conjugated

MBS716030-5x01mg 5x0.1mg
EUR 1205

Cancer/Testis Antigen 5.1 (SSX1) Antibody (Biotin)

20-abx316208
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Cancer/Testis Antigen 5.1 (SSX1) Antibody (Biotin)

abx316208-100g 100 µg
EUR 362.5

Cancer/Testis Antigen 5.1 (SSX1) Antibody (Biotin)

abx316208-20g 20 µg
EUR 162.5

Cancer/Testis Antigen 5.1 (SSX1) Antibody (Biotin)

abx316208-50g 50 µg
EUR 250

Human Sialylated Lewis A Antigen (CA 19-9) Antibody (Biotin Conjugate)

30261-05121 150 ug
EUR 315

OASA08742-1ML - Salmonella Group Antigen Antibody - Biotin Conjugated

OASA08742-1ML 1ml
EUR 479

Cancer Antigen CA19-9 (Gastrointestinal Cancer) (Biotin)

MBS6127297-01mL 0.1(mL
EUR 1060

Cancer Antigen CA19-9 (Gastrointestinal Cancer) (Biotin)

MBS6127297-5x01mL 5x0.1mL
EUR 4615

Cancer Antigen CA19-9 (Gastrointestinal Cancer) (Biotin)

MBS6127298-01mL 0.1(mL
EUR 1060

Cancer Antigen CA19-9 (Gastrointestinal Cancer) (Biotin)

MBS6127298-5x01mL 5x0.1mL
EUR 4615

Cancer Antigen CA19-9 (Gastrointestinal Cancer) (Biotin)

MBS6127299-01mL 0.1(mL
EUR 1060

Cancer Antigen CA19-9 (Gastrointestinal Cancer) (Biotin)

MBS6127299-5x01mL 5x0.1mL
EUR 4615

Rabbit anti-Mouse CD63 antigen polyclonal Antibody, Biotin conjugated

MBS1488249-005mg 0.05mg
EUR 190

Rabbit anti-Mouse CD63 antigen polyclonal Antibody, Biotin conjugated

MBS1488249-01mg 0.1mg
EUR 270

Rabbit anti-Mouse CD63 antigen polyclonal Antibody, Biotin conjugated

MBS1488249-5x01mg 5x0.1mg
EUR 1205

Rabbit anti-mouse CD82 antigen polyclonal Antibody, Biotin conjugated

MBS1491466-005mg 0.05mg
EUR 190

Rabbit anti-mouse CD82 antigen polyclonal Antibody, Biotin conjugated

MBS1491466-01mg 0.1mg
EUR 270

Rabbit anti-mouse CD82 antigen polyclonal Antibody, Biotin conjugated

MBS1491466-5x01mg 5x0.1mg
EUR 1205

CA19-9 (Cancer, Cancer antigen 19-9, CA199) (Biotin)

MBS6261468-01mL 0.1mL
EUR 1085

CA19-9 (Cancer, Cancer antigen 19-9, CA199) (Biotin)

MBS6261468-5x01mL 5x0.1mL
EUR 4725

CA125 Cancer Antigen, A Domain (Ovarian Cancer) (Biotin)

MBS6127289-01mL 0.1(mL
EUR 1350

CA125 Cancer Antigen, A Domain (Ovarian Cancer) (Biotin)

MBS6127289-5x01mL 5x0.1mL
EUR 5920

CA125 Cancer Antigen, B Domain (Ovarian Cancer) (Biotin)

MBS6127290-01mL 0.1(mL
EUR 1350

CA125 Cancer Antigen, B Domain (Ovarian Cancer) (Biotin)

MBS6127290-5x01mL 5x0.1mL
EUR 5920

Carbohydrate Antigen 125 (CA125) Antibody (Biotin)

20-abx272665
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Carbohydrate Antigen 125 (CA125) Antibody (Biotin)

20-abx271489
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Carbohydrate Antigen 125 (CA125) Antibody (Biotin)

abx271489-1ml 1 ml
EUR 737.5

Carbohydrate Antigen 125 (CA125) Antibody (Biotin)

abx271489-200l 200 µl
EUR 337.5

Carbohydrate Antigen 125 (CA125) Antibody (Biotin)

abx272665-100l 100 µl
EUR 337.5

Carbohydrate Antigen 125 (CA125) Antibody (Biotin)

abx272665-1ml 1 ml Ask for price

Carbohydrate Antigen 125 (CA125) Antibody (Biotin)

abx272665-200l 200 µl
EUR 762.5

Rabbit anti-human G antigen 5 polyclonal Antibody, Biotin conjugated

MBS1488890-005mg 0.05mg
EUR 190

Rabbit anti-human G antigen 5 polyclonal Antibody, Biotin conjugated

MBS1488890-01mg 0.1mg
EUR 270

Rabbit anti-human G antigen 5 polyclonal Antibody, Biotin conjugated

MBS1488890-5x01mg 5x0.1mg
EUR 1205

Cancer Antigen CA 19-9 Antibody

abx023798-1mg 1 mg
EUR 1053.6

Rabbit anti-human Antigen KI-67 polyclonal Antibody, Biotin conjugated

MBS1488970-005mg 0.05mg
EUR 190

Rabbit anti-human Antigen KI-67 polyclonal Antibody, Biotin conjugated

MBS1488970-01mg 0.1mg
EUR 270

Rabbit anti-human Antigen KI-67 polyclonal Antibody, Biotin conjugated

MBS1488970-5x01mg 5x0.1mg
EUR 1205

CA125 Cancer Antigen (Ovarian Cancer) (Glycerol Free) (Biotin)

MBS6261276-01mL 0.1mL
EUR 970

CA125 Cancer Antigen (Ovarian Cancer) (Glycerol Free) (Biotin)

MBS6261276-5x01mL 5x0.1mL
EUR 4220

Cancer Antigen 50 (CA 50), Antibody

GWB-737484 1 mg Ask for price

MUC16 (Mucin-16, MUC-16, CA-125, Ovarian Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125, CA125, FLJ14303) (Biotin)

MBS6261406-01mL 0.1mL
EUR 970

MUC16 (Mucin-16, MUC-16, CA-125, Ovarian Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125, CA125, FLJ14303) (Biotin)

MBS6261406-5x01mL 5x0.1mL
EUR 4220

MUC16 (Mucin-16, MUC-16, CA-125, Ovarian Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125, CA125, FLJ14303) (Biotin)

MBS6261407-01mL 0.1mL
EUR 970

MUC16 (Mucin-16, MUC-16, CA-125, Ovarian Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125, CA125, FLJ14303) (Biotin)

MBS6261407-5x01mL 5x0.1mL
EUR 4220

MUC16 (Mucin-16, MUC-16, CA-125, Ovarian Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125, CA125, FLJ14303) (Biotin)

MBS6261408-01mL 0.1mL
EUR 920

MUC16 (Mucin-16, MUC-16, CA-125, Ovarian Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125, CA125, FLJ14303) (Biotin)

MBS6261408-5x01mL 5x0.1mL
EUR 3980

MUC16 (Mucin-16, MUC-16, CA-125, Ovarian Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125, CA125, FLJ14303) (Biotin)

MBS6261409-01mL 0.1mL
EUR 1095

MUC16 (Mucin-16, MUC-16, CA-125, Ovarian Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125, CA125, FLJ14303) (Biotin)

MBS6261409-5x01mL 5x0.1mL
EUR 4785

Rabbit anti-Toxocara canis 26 kDa secreted antigen polyclonal Antibody, Biotin conjugated

MBS1493177-005mg 0.05mg
EUR 190

Rabbit anti-Toxocara canis 26 kDa secreted antigen polyclonal Antibody, Biotin conjugated

MBS1493177-01mg 0.1mg
EUR 270

To our knowledge, this is the first validation total RNA sequencing archived FFPE material provide a reliable estimate of the level of the marker protein. These results suggest that in the future, RNA sequencing can equip immunohistochemistry for the reliable measurement of biomarker expression in FFPE cancer samples.