Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naïve/Memory T Cell Immunomonitoring.
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Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naïve/Memory T Cell Immunomonitoring.
Personalized medicine in oncology needs immunological test standards. Flow cytometry (FCM) method is an important tool for immunomonitoring, and their harmonization is essential to obtain comparable data in multicenter clinical trials. The purpose of this study was to design a workflow harmonization can address the problems most effectively contribute to intra- and inter-operator variability in project.
The multicenter Italian National Institute of Health (Istituto Superiore di Sanità, ISS) succeeded in multiparametric flow cytometric panel harmonization between thirteen owned operator of five clinical centers and research areas Lazio (Italy). panel based on a mixture backbone of antibodies dried (anti-CD3, anti-CD4, anti-CD8, anti-CD45RA and anti-CCR7) for detecting cell / memory T naive, is recognized as a potential prognostic / predictive immunological biomarkers in immunotherapies of cancer.
The coordination center congealed distributed peripheral blood mononuclear cells (PBMC) and a fresh sample of whole blood (WB) from a healthy donor, reagents and standard operating procedures (SOP) for participants to experiment with their own equipment, to simulate a real-life scenario. Operators and local back raw data were analyzed for the ISS for central analysis and statistics elaboration.Harmonised and reproducible results are obtained by sharing the experimental set-up and procedures along with the centralization of data analysis, which leads to a reduction in cross-center variability naive / memory section in particular frequency whole blood setting.
Our experimental and analytical work processes proved to be suitable for harmonization of FCM test in a multicenter setting, where high quality data needed to evaluate the potential for immunological markers, which can contribute to choose a better therapeutic selection.
Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naïve/Memory T Cell Immunomonitoring.
RNAseq in comparison to Immunohistochemistry to Measure Cancer Biomarkers in Breast Cancer and Lung Cancer Specimens.
RNA sequencing is considered the gold standard for high-throughput gene expression profile at the level of transcription. increasingly important in cancer research and molecular diagnostics is reflected in the growing number of mentions in the scientific literature and clinical trials reports. However, the use of different reagents and protocols for RNA sequencing often produce results that are not compatible.
More recently, we published a profile of Atlas Oncobox RNA sequencing to normal human tissues were obtained from a healthy donor were killed in road accidents. This is a database of molecular profiles obtained using the setting uniform protocol and reagents that can be widely used in biomedicine for the normalization of data in pathology, including cancer.
Here, we publish 39 original new breast cancer (BC) and the profile 19 of lung cancer (LC) RNA sequencing was obtained for paraffin-embedded samples (FFPE) tissue formalin-fixed, fully compatible with Oncobox Atlas. We did the first correlation study RNA sequencing and immunohistochemical expression profile-measured for actionable clinical biomarker genes in the cancer FFPE tissue samples. We showed a high (Spearman’s rho 0.65 to 0.798) and statistically significant (p <0.00004) correlation between RNA sequencing (Oncobox protocol) and immunohistochemical measurement of HER2 / ERBB2, ER / ESR1 and PGR gene in BC, and for gene PDL1 The LC; AUC: 0.963 to HER2, 0.921 for ESR1, 0.912 to PGR, and 0.922 for PDL1.
Description: A polyclonal antibody against CANX. Recognizes CANX from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against CANT1. Recognizes CANT1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against CAND1. Recognizes CAND1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Rat Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Ovarian cancer marker/Carbohydrate antigen 125 ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Ovarian cancer marker/Carbohydrate antigen 125 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human T-Cell Antigen CD7 (CD7) Antibody (Biotin Conjugate)
Description: A polyclonal antibody against EGM_08965. Recognizes EGM_08965 from Macaca fascicularis. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against I79_001071. Recognizes I79_001071 from Cricetulus griseus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against PG_1424. Recognizes PG_1424 from Porphyromonas gingivalis. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against CS. Recognizes CS from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against TG. Recognizes TG from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against KL. Recognizes KL from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against CP. Recognizes CP from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against HP. Recognizes HP from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against G. Recognizes G from Rabies virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against AR. Recognizes AR from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against C5. Recognizes C5 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against C6. Recognizes C6 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against C9. Recognizes C9 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against gD. Recognizes gD from Human herpesvirus 1. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against HA. Recognizes HA from Influenza A virus(H7N9). This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against th. Recognizes th from Zebrafish. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against L1. Recognizes L1 from Human papillomavirus type 16. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against L1. Recognizes L1 from Human papillomavirus type 18. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against C3. Recognizes C3 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against GK. Recognizes GK from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against IX. Recognizes IX from Enterobacteria phage M13. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against P. Recognizes P from Rabies virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against GP. Recognizes GP from Zaire ebolavirus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against GP. Recognizes GP from Zaire ebolavirus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against GP. Recognizes GP from Zaire ebolavirus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against F. Recognizes F from Canine distemper virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against N. Recognizes N from Lassa virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against N. Recognizes N from Junin mammarenavirus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against gH. Recognizes gH from Human cytomegalovirus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against N. Recognizes N from Rabies virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against N. Recognizes N from Rabies virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against M. Recognizes M from Rabies virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against S. Recognizes S from Bovine coronavirus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against C. Recognizes C from Rabies virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against X. Recognizes X from Woodchuck hepatitis B virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against P. Recognizes P from Hepatitis B virus genotype A2 subtype adw2. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against GC. Recognizes GC from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against MB. Recognizes MB from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against X. Recognizes X from Hepatitis B virus genotype D subtype ayw. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against NP. Recognizes NP from Reston ebolavirus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against B1. Recognizes B1 from Human herpesvirus 6B. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against L. Recognizes L from Vesicular stomatitis Indiana virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against FH. Recognizes FH from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against PC. Recognizes PC from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against XG. Recognizes XG from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against F2. Recognizes F2 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against F3. Recognizes F3 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against F5. Recognizes F5 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against F7. Recognizes F7 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against F8. Recognizes F8 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against F9. Recognizes F9 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against TH. Recognizes TH from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against TK. Recognizes TK from Human herpesvirus 1. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against MB. Recognizes MB from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against AK. Recognizes AK from Penaeus monodon. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against gL. Recognizes gL from Human cytomegalovirus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against X. Recognizes X from Hepatitis B virus genotype D. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against NP. Recognizes NP from Influenza A virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against P. Recognizes P from Vesicular stomatitis Indiana virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against NS. Recognizes NS from Influenza A virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against HA. Recognizes HA from Influenza A virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against NP. Recognizes NP from Influenza A virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against S. Recognizes S from Bovine coronavirus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against E6. Recognizes E6 from Human papillomavirus type 52. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against N. Recognizes N from Puumala virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against S. Recognizes S from Human coronavirus OC43. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against GP. Recognizes GP from Zaire ebolavirus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against X. Recognizes X from Hepatitis B virus genotype D subtype ayw. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against F. Recognizes F from Human respiRatory syncytial virus A. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against NP. Recognizes NP from Influenza A virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against NS. Recognizes NS from Influenza A virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against HA. Recognizes HA from Influenza A virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against NP. Recognizes NP from Influenza B virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against L2. Recognizes L2 from Human papillomavirus type 18. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against NP. Recognizes NP from Influenza B virus. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against gG. Recognizes gG from Human herpesvirus 1. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against E7. Recognizes E7 from Human papillomavirus type 18. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against E2. Recognizes E2 from Human papillomavirus type 18. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against gB. Recognizes gB from Human herpesvirus 2. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against C3. Recognizes C3 from Mouse. This antibody is Biotin conjugated. Tested in the following application: ELISA
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To our knowledge, this is the first validation total RNA sequencing archived FFPE material provide a reliable estimate of the level of the marker protein. These results suggest that in the future, RNA sequencing can equip immunohistochemistry for the reliable measurement of biomarker expression in FFPE cancer samples.