Simplified low-cost methodology to establish, histologically process and analyze three-dimensional cancer cell spheroid arrays

Different from the two-dimensional cell culture, three-dimensional (3D) model of multicellular spheroid allows cells to build interactions cell-cell / cell-matrix on the surface of cells throughout, more closely mimic microenvironments of tumors and a subpopulation cell with a certain standard morphology, differentiation and expression gen.

Since then several methodologies that involve or aggregate 3D generation cells or histological processing and analysis have appeared, but in general they are laborious, expensive and complicated to set up as a routine technique. Thus, we developed a complete methodology, detailing simple, accessible and inexpensive step by step, including 1) 3D aggregate generation cells using the technique of reduction depends; 2) provide a simple way to assess the morphological parameters of spheroids produced; followed by 3) multiples and organized histological processing, keeping some individual spheroids in an agarose tool, maintaining order is known and the position of each, similar to a tissue microarray principle; 4) until the last step, in which he allowed the simultaneous histological analysis of the composition of a few slices of round, well-organized side by side, in the same block, through conventional staining or 5) immunostaining against different molecular markers.

Therefore, the purpose of present methodology for the popularization of 3D cell culture, enabling to make these unusual techniques in basic cell biology research, after all the steps performed without the use of heavy reagents, materials or equipment. Besides bringing the apparatus simple agarose as new low cost, which allows high-throughput analysis of multiple spheroids simultaneously in an organized manner.

 Simplified low-cost methodology to establish, histologically process and analyze three-dimensional cancer cell spheroid arrays
Simplified low-cost methodology to establish, histologically process and analyze three-dimensional cancer cell spheroid arrays

Synthesis and Evaluation of Some Novel Antimicrobial pyrimidine, pyrazole, Chromene and Tetrahydrobenzo [b] thiophene derivatives Bearing Pyrimidinthione part

Aims and objectives: A collection of novel fused pyrimidine, pyridine, pyrazole, chromene, thiophene derivatives 2-30 has been newly synthesized by using 1a, b as the starting material. Fused Pyrans show off a variety of pharmacological activities such as cancer agents [1], antimicrobial [2-4], antioxidants [5], the anti-proliferation [6], cytotoxic activity [7], the anticipated anti-tumor [8], antiparkinson [9] and anti-inflammatory [10]. Also, derivatives Pyrans famous for bacterial biofilm disruptor [11], anticonvulsants [12] and inhibtors of mycobacterium bovis [13].


Materials and methods: All melting points are measured using Block Akofler instrument and are uncorrected. IR spectrum (KBr) recorded in 5300 FTIR spectrometer (υ, cm-1). 1H-NMR were recorded on a Varian Gemini spectrometer. 1H-NMR run at 300, 400 MHz and 13 C-NMR run at 100 MHz in DMSO-d6, CDCl3 as solvent.

Chemical shifts expressed in parts per million (ppm) using tetramethylsilane (TMS) as an internal refernce. 1000 EX mass spectrometer at 70 eV. The purity of the synthesized compounds is checked by thin layer chromatography (TLC) (aluminum sheet) using n-hexane, EtOAc (9: 1, V / V, 7: 3 V / V) eluent. Elemental analysis performed by the microanalytical Research Center, Faculty of Science, and microanalytical Unit, Faculty of Pharmacy, Cairo University, Egypt.

Human Cancer Antigen 15-3 (CA 15-3) ELISA Kit

PRB-5069-5 5 x 96 assays
EUR 2283

Cancer Antigen CA 15-3 Protein (Human Fluids)

VAng-Wyb8628-inquire inquire Ask for price
Description: CA 15-3 (Breast Cancer Antigen), Cancer antigen from Human Fluids, 12.240 kU/mL.

Cancer Antigen CA 15-3 Protein (Human Milk)

VAng-Wyb8631-inquire inquire Ask for price
Description: CA 15-3 (Breast Cancer Antigen), Antigen Grade, Cancer antigen from Human Milk, 33.0 kIU/mL.

99445-15 DCT 15 X 85MM

99445-15 250/pk
EUR 97
Description: Disposable Culture Tubes; DCT's, CGW

9998 SCREW CAP 415/15

9998-15 288/pk
EUR 198
Description: General Apparatus; Stoppers

CA 15-3 antibody

10-1144 250 ug
EUR 296
Description: Mouse monoclonal CA 15-3 antibody

CA 15-3 antibody

10-1145 1 mg
EUR 674
Description: Mouse monoclonal CA 15-3 antibody

CA 15-3 antibody

10-1146 250 ug
EUR 219
Description: Mouse monoclonal CA 15-3 antibody

CA 15-3 antibody

10-2344 1 mg
EUR 457
Description: Mouse Monoclonal CA 15-3 antibody

CA 15-3 antibody

10-C03E 1 mg
EUR 300
Description: Mouse monoclonal CA 15-3 antibody

CA 15-3 antibody

10-C03F 1 mg
EUR 300
Description: Mouse monoclonal CA 15-3 antibody

CA 15-3 antibody

10-CA15A 1 mg
EUR 565
Description: Mouse monoclonal CA 15-3 antibody

CA 15-3 antibody

10-CA15B 1 mg
EUR 565
Description: Mouse monoclonal CA 15-3 antibody

CA 15-3 antibody

10-CA15C 1 mg
EUR 565
Description: Mouse monoclonal CA 15-3 antibody

CA 15-3 antibody

10-CA15D 1 mg
EUR 565
Description: Mouse monoclonal CA 15-3 antibody

CA 15-3 protein

30C-CP1064 10 KU
EUR 301
Description: Partially purified Human CA 15-3 protein

CA 15-3 protein

30C-CP9064U 10 KU
EUR 570
Description: Highly pure Human CA 15-3 protein

CA 15-3 protein

30-1066 1 kU
EUR 127
Description: Purified native Human CA 15-3 protein

CA 15-3 protein

30-AC12 25 KU
EUR 457
Description: Partially purified Human CA 15-3 protein

CA 15-3 protein

30-AC16S 25 KU
EUR 511
Description: Partially purified Human CA 15-3 protein

CA 15-3 protein

30-AC16S2 25 KU
EUR 673
Description: Partially pure native Human CA 15-3 protein

Human Carbohydrate Antigen 15-3 (CA15-3) ELISA Kit

DLR-CA15-3-Hu-48T 48T
EUR 479
  • Should the Human Carbohydrate Antigen 15-3 (CA15-3) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Carbohydrate Antigen 15-3 (CA15-3) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Carbohydrate Antigen 15-3 (CA15-3) ELISA Kit

DLR-CA15-3-Hu-96T 96T
EUR 621
  • Should the Human Carbohydrate Antigen 15-3 (CA15-3) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Carbohydrate Antigen 15-3 (CA15-3) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Carbohydrate Antigen 15-3 (CA15-3) ELISA Kit

RD-CA15-3-Hu-48Tests 48 Tests
EUR 478

Human Carbohydrate Antigen 15-3 (CA15-3) ELISA Kit

RD-CA15-3-Hu-96Tests 96 Tests
EUR 662

Human Carbohydrate Antigen 15-3 (CA15-3) ELISA Kit

RDR-CA15-3-Hu-48Tests 48 Tests
EUR 500

Human Carbohydrate Antigen 15-3 (CA15-3) ELISA Kit

RDR-CA15-3-Hu-96Tests 96 Tests
EUR 692

Swine IL-15 Recombinant Protein

R00212-3 5ug/vial
EUR 259
Description: Interleukin-15 (IL-15) is a cytokine with structural similarity to IL-2 that is secreted by mononuclear phagocytes following infection by virus(es). This cytokine induces cell proliferation of natural killer cells. Swine IL-15 Recombinant Protein is purified interleukin-15 produced in yeast.

Recombinant Human IL-15 Protein

PROTP40933-3 10ug
EUR 317
Description: IL-15 is an immunomodulating cytokine that stimulates the proliferation of T lymphocytes and shares many biological properties with IL-2. IL-15 exerts its biological activites primarily on T cells. It is also essential in the development, survival and activation of NK cells. Increased expression of IL-15 has been implicated with rheumatoid arthritis, inflammatory bowel disease and diseases affiliated with retroviruses HIV and HTLV-I. Human IL-15 is biologically active on mouse cells as measured by the dose-dependent stimulation of the proliferation of mouse CTLL cells. Recombinant human IL-15 is a 12.9 kDa protein consisting of 114 amino acid residues.

AXYGEN® HORIZONTAL GEL BOX, 15 CM

HGB-15 1/pk
EUR 486
Description: Lab Equipment; Axygen Branded EQ

DiagNano Fluorophore Labeled Gold Nanoparticles, 15 nm

GFL-15 1 mL
EUR 1053

CA 15-3 antibody (HRP)

61-CA15BHRP 500 ul
EUR 349
Description: Mouse monoclonal CA 15-3 antibody (HRP)

CA 15-3 ELISA kit

55R-IB19123 96 wells
EUR 440
Description: ELISA kit for the detection of CA 15-3 in the research laboratory

Gastrointestinal Cancer Antigen (CA 19-9), antigen grade

CA1991-N-10 10 KU
EUR 529

AXYGEN® GEL TRAY 15 X 15 CM FOR USE WITH 15 CM GEL BOX, UV TRANSPARENT

HGB15-15-GT 1/pk
EUR 79
Description: Lab Equipment; Axygen Branded EQ

DiagNano Gold Nanoparticle Passive Conjugation Kit, 15 nm

GPK-15 1 kit
EUR 715

Cancer Antigen 15-3 MUC 1 Antigen (Human Milk)

VAng-Cr3871-10kU 10 kU
EUR 421
Description: Cancer Antigen 15-3 MUC 1 Antigen, Host/Source: Human Milk. The purity is detected by salt extraction and delipidization following high speed centrifugation. It is tested negative for HBsAg, antibodies to HCV and HIV 1/2.

Cancer Antigen 15-3 (CA15-3) (human) ELISA Kit

K4804-100 100 assays
EUR 834
  • Kit components:
  • CA15-3 Ab-coated Microplate (Item A), 96 wells
  • Wash Buffer (20x) (Item B)
  • Human CA15-3 Standard (Item C)
  • Assay Diluent A (Item D)
  • Assay Diluent B (5x) (Item E)
  • Detection Antibody (Item F)
  • HRP-Streptavidin (200x) (Item
  • Show more
Description: Sensitive, Colorimetric Assay

Cancer Antigen 15-3 MUC 1 Antigen (Human Ascites Fluid)

VAng-Cr3872-10kU 10 kU
EUR 1136
Description: Cancer Antigen 15-3 MUC 1 Antigen, Host/Source: Human Ascites Fluid.

RealScreen CA 15-3 ELISA Kit

EL067-096 96T
EUR 968

CA 15-3 High Pure Protein

abx061025-10kU 10 kU
EUR 1929
  • Shipped within 5-10 working days.

TM-CA 15-3 ELSIA Kit

DEIA2145 96T
EUR 655
Description: The TM-CA 15-3 ELISA is an enzyme immunoassay for measurement of CA15-3 in serum and plasma.

CA 15-3, Human Breast Adenocarcinoma

P1435-1
EUR 109

CA 15-3, Human Breast Adenocarcinoma

P1435-5
EUR 240

DiagNano Fluorophore Labeled Gold Nanoparticles, 15 nm, Cellular Uptake

GFL-15-CU 1mL
EUR 1365

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 15 nm

GCK-M-15 1 kit
EUR 757

Recombinant CA 15-3 Protein (Cell Culture Supernatant)

VAng-Wyb8629-inquire inquire Ask for price
Description: CA 15-3 (Breast Cancer Antigen), Calibrator Grade, recombinant protein from Cell Culture Supernatant, 10003 Units/mL.

Ovarian Cancer Antigen (CA 125)

CA1251-N-10 10 KU
EUR 529

TruStrip Sample Transfer Strips, 15-ul, 50/Pk (without sample tracking dye)

STS-15-50 1 Pk Ask for price

TruStrip Sample Transfer Strips, 15-ul, 50/Pk (with sample tracking dye)

STSD-15-50 1 Pk Ask for price

Human Mucin-1 (MUC-1, CA 15-3) Antibody

32188-05111 150 ug
EUR 261

Cancer Antigen CA-125 Antigen (Human Fluids)

VAng-Wyb8639-inquire inquire Ask for price
Description: CA-125, Calibrator Grade, cancer antigen from Human Fluids, 53.26 KU/mL.

Cancer Antigen CA-125 Antigen (Human Adenocarcinoma)

VAng-Wyb8642-inquire inquire Ask for price
Description: CA-125, Calibrator Grade, Cancer antigen from Human Adenocarcinoma, 500 kIU / mL.

Paraffin Tissue Section - Human Brain Tumor: Astrocytoma Grade III

T2235035-3 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

TP62(47&15) Antigen

E62H00703 100ug
EUR 382

AXYGEN® 10-WELL COMB FOR USE WITH 15 CM GEL BOX, 1.5 MM THICKNESS

HGB15-10-15 1/pk
EUR 60
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 12-WELL COMB FOR USE WITH 15 CM GEL BOX, 1.5 MM THICKNESS

HGB15-12-15 1/pk
EUR 60
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 20-WELL COMB FOR USE WITH 15 CM GEL BOX, 1.5 MM THICKNESS

HGB15-20-15 1/pk
EUR 60
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 35-WELL COMB FOR USE WITH 15 CM GEL BOX, 1.5 MM THICKNESS

HGB15-35-15 1/pk
EUR 69
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 8-WELL COMB FOR USE WITH 15 CM GEL BOX, 1.5 MM THICKNESS

HGB15-8-15 1/pk
EUR 69
Description: Lab Equipment; Axygen Branded EQ

Mouse Carbohydrate Antigen 15-3(CA15-3)ELISA kit

GA-E0825MS-48T 48T
EUR 336

Mouse Carbohydrate Antigen 15-3(CA15-3)ELISA kit

GA-E0825MS-96T 96T
EUR 534

Rat CarbohydRate Antigen 15-3(CA15-3)ELISA kit

GA-E0858RT-48T 48T
EUR 354

Rat CarbohydRate Antigen 15-3(CA15-3)ELISA kit

GA-E0858RT-96T 96T
EUR 571

Rat CarbohydRate Antigen 15-3(CA15-3)ELISA kit

QY-E10794 96T
EUR 361

Mouse CarbohydMousee Antigen 15-3(CA15-3)ELISA kit

QY-E20245 96T
EUR 361

Cancer Antigen CA 19-9 Antibody

abx023798-1mg 1 mg
EUR 878
  • Shipped within 5-10 working days.

Recombinant CA 15-3 Protein (Human BTA Cell Line Supernatant)

VAng-Wyb8630-inquire inquire Ask for price
Description: CA 15-3 (Breast Cancer Antigen), Antigen Grade, recombinant protein from Human BTA Cell Line Supernatant, 20171 Units/mL.

T3 (Triiodothyronine) ELISA test

15 96T/Box Ask for price
  • Area of application: Hormone testing
Description: ELISA based test for quantitative detection of T3 (Triiodothyronine)

3-Dehydro-15-deoxoeucosterol

TB01583 5mg
EUR 847

15-Methoxyansamitocin P-3

TBZ0596 unit Ask for price

AXYGEN® 14-WELL MULTI-CHANNEL COMPATIBLE COMB FOR USE WITH 15 CM GEL BOX, 1.5 MM THICKNESS

HGB15-14MC-15 1/pk
EUR 63
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 16-WELL MULTI-CHANNEL COMPATIBLE COMB FOR USE WITH 15 CM GEL BOX, 1.5 MM THICKNESS

HGB15-16MC-15 1/pk
EUR 60
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 18-WELL MULTI-CHANNEL COMPATIBLE COMB FOR USE WITH 15 CM GEL BOX, 1.5 MM THICKNESS

HGB15-18MC-15 1/pk
EUR 60
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 28-WELL MULTI-CHANNEL COMPATIBLE COMB FOR USE WITH 15 CM GEL BOX, 1.5 MM THICKNESS

HGB15-28MC-15 1/pk
EUR 62
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 30-WELL MULTI-CHANNEL COMPATIBLE COMB FOR USE WITH 15 CM GEL BOX, 1.5 MM THICKNESS

HGB15-30MC-15 1/pk
EUR 62
Description: Lab Equipment; Axygen Branded EQ

IL-3 Interleukin-3 Human Recombinant Protein, His Tag

PROTP08700-3 Regular: 50ug
EUR 317
Description: Interleukin-3 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 154 amino acids fragment (20-152) and having a total molecular mass of 17.3kDa and fused with a 20 aa N-terminal His tag. ;The IL3 His is purified by proprietary chromatographic techniques.

Anti-14-3-3 alpha + beta Rabbit Monoclonal Antibody

M02431-3 100ug/vial
EUR 397
Description: Rabbit Monoclonal 14-3-3 alpha + beta Antibody. Validated in Flow Cytometry, IP, IF, IHC, ICC, WB and tested in Human, Mouse, Rat.

Polyoxyl 15 Hydroxystearate USP, Ph. Eur. grade

GX7347-100G 100 g
EUR 61

Polyoxyl 15 Hydroxystearate USP, Ph. Eur. grade

GX7347-1KG 1 kg
EUR 175

Polyoxyl 15 Hydroxystearate USP, Ph. Eur. grade

GX7347-500G 500 g
EUR 110

Individual Reaction Mix 3

G065-3 200 reactions
EUR 167

3-D Life Thioglycerol

T10-3 180 µl
EUR 48

Western Blot Box - 2 7/8 x 1 3/16 x 3/4in.; 7.3 x 3 x 1.9cm

B1200-3 5/pack
EUR 63.77
Description: Western Blot Boxes

Human Cancer Tissue Preparation Buffer 15: Skin Cancer Cells

9-80038 1 x 100 ml Ask for price

Human Mucin-1 (MUC-1, CA 15-3) Antibody (Biotin Conjugate)

32188-05121 150 ug
EUR 369

TGF-b-3 Transforming Growth Factor-Beta 3 Human Recombinant Protein, Plant

PROTP10600-3 Regular: 5ug
EUR 317
Description: TGFB3 Human Recombinant produced in plant is a disulfide-linked homodimeric, glycosylated, polypeptide chain containing 118 amino acids and having a molecular mass of 27.2kDa. ;The TGFB3 is fused to 6xHis tag at N-terminus and purified by standard chromatographic techniques.

Cancer Antigen CA-125 Antigen (Human Fluids) (Partially Pure)

VAng-Wyb8637-inquire inquire Ask for price
Description: CA-125 (Ovarian Cancer), Partially Pure, Cancer antigen from Human Fluids, 73.90 kUnits/mL.

Cancer Antigen CA-125 Antigen (Ovarian Carcinoma Cell Line)

VAng-Wyb8641-inquire inquire Ask for price
Description: CA-125, Antigen Grade, Cancer antigen from Ovarian Carcinoma Cell Line, 84,040 Units/mL.

Dr. P Kit-Solution 3

K2021010-3 50 ml
EUR 133
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

3-D Life RGD Peptide

P10-3 3x 1 µmol
EUR 276

Recombinant Human MMP-3 Protein

PROTP08254-3 10ug
EUR 317
Description: Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-3 degrades fibronectin, laminin, collagens III, IV, and X, and cartilage proteoglycans. Recombinant human MMP-3 is a 42.8 kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain (378 amino acids).

3-D-Life SG-PVA

M81-3 3x 170 µl
EUR 148

3-D Life FG-PVA

M82-3 3x 170 µl
EUR 148

3-D Life SG-Dextran

M91-3 3x 170 µl
EUR 157

3-D Life FG-Dextran

M92-3 3x 170 µl
EUR 157

3-D Life PEG-Link

L50-3 3x 200 µl
EUR 94

3-D Life CD-Link

L60-3 3 x 200 µl
EUR 321

pLenti-CARM1 shRNA-3 Plasmid

PVTBAV03691-3 2 ug
EUR 356

pLenti-CTLA4 shRNA-3 Plasmid

PVTBAV05689-3 2 ug
EUR 356

pLenti-FOXM1 shRNA-3 Plasmid

PVTBAV08732-3 2 ug
EUR 356

pLenti-JUN shRNA-3 Plasmid

PVTBAV11741-3 2 ug
EUR 356

pLenti-LHX6 shRNA-3 Plasmid

PVTBAV12881-3 2 ug
EUR 356

pLenti-MAGEA3 shRNA-3 Plasmid

PVTBAV13661-3 2 ug
EUR 356

pLenti-RUNX3 shRNA-3 Plasmid

PVTBAV20583-3 2 ug
EUR 356

pLenti-Slc7a11 shRNA-3 Plasmid

PVTBAV21973-3 2 ug
EUR 356

pLenti-STAT3 shRNA-3 Plasmid

PVTBAV22921-3 2 ug
EUR 356

Recombinant (E. coli) Dengue Virus Type 3 E Antigen (DENV-E), antigen grade (>95%, 15 kda, no tag)

RP-1596 50 ug
EUR 408

Inactivated CMV Grade 3 Antigen

VAng-Lsx0038-1mg 1 mg
EUR 2086
Description: CMV Grade 3, natural protein.

Inactivated MuV Grade 3 Antigen

VAng-Lsx0411-inquire inquire Ask for price
Description: MuV grade 3, native protein.

Inactivated Rotavirus Grade 3 Antigen

VAng-Lsx0469-inquire inquire Ask for price
Description: Rotavirus Grade 3, native virus.

Inactivated RuV Grade 3 Antigen

VAng-Lsx0476-1mL 1 mL
EUR 395
Description: RuV Grade 3, natural protein.

FSH (Human Follicle-stimulating hormone) ELISA test

3 96T/Box Ask for price
  • Area of application: Hormone testing
Description: ELISA based test for quantitative detection of FSH (Human Follicle-stimulating hormone)

Anti-VEGF Receptor 3/FLT4 Antibody

A01276-3 100ug/vial
EUR 334

Caspase-3 Inhibitor Q-DEVD-OPh

1175-3
EUR 533

3-D Life Scrambled RGD Peptide

P11-3 3x 1 µmol
EUR 276

Anti-active Caspase-3 Rabbit Monoclonal Antibody

M00334-3 100ug/vial
EUR 397
Description: Anti-active Caspase-3 Rabbit Monoclonal Antibody tested for IF, IHC, ICC, WB in Human

Anti-Galectin 3/LGALS3 Antibody (monoclonal, 12B12)

M00621-3 100ug/vial
EUR 334

QuantiMir 3'-Universal Primer (10,000 qPCR reactions)

RA420AU-3 10,000 assays
EUR 178
  • Category: MicroRNA Tools

SeraMir 3'-Universal Primer (10,000 qPCR reactions)

RA800P-3 10,000 reactions
EUR 178
  • Category: MicroRNA Tools

Human Mucin-1 (MUC-1, CA 15-3) AssayLite Antibody (FITC Conjugate)

32188-05141 150 ug
EUR 428

Human Mucin-1 (MUC-1, CA 15-3) AssayLite Antibody (RPE Conjugate)

32188-05151 150 ug
EUR 428

Human Mucin-1 (MUC-1, CA 15-3) AssayLite Antibody (APC Conjugate)

32188-05161 150 ug
EUR 428

Human Mucin-1 (MUC-1, CA 15-3) AssayLite Antibody (PerCP Conjugate)

32188-05171 150 ug
EUR 471

3-D Life 10x CB Buffer (pH 7.2)

B20-3 600 µL
EUR 44

3-D Life 10x CB-Buffer (pH 5.5)

B10-3 600 µl
EUR 44

Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit

30009-3 1KIT
EUR 809
Description: Minimum order quantity: 1 unit of 1KIT

Anti-Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Monoclonal Antibody

M00227-3 100ul
EUR 397
Description: Mouse Monoclonal Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Antibody. Validated in IHC, WB and tested in Equine.

Interleukin 15 (IL-15) Antibody

abx432855-200ul 200 ul
EUR 384
  • Shipped within 1-3 working days.

Cytokeratin 15 (Cytokeratin 15) Antibody

20-abx225265
  • EUR 370.00
  • EUR 606.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 50 ul
  • Shipped within 5-10 working days.

Cytokeratin 15 (Cytokeratin 15) Antibody

abx232198-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.

Cytokeratin 15 (Cytokeratin 15) Antibody

abx232199-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.


Results: A series of novel azoles and azines designed and prepared by reacting 7-amino-5- (4-chlorophenyl) – 4-phenyl-2-thioxo-2,5-dihydro – 1H pyrano- [2, 3-d] pyrimidine -6-carbonitrile 1a and 7-amino-4,5-diphenyl-2-thioxo-2,5- dihydro-1H-pyrano [2,3-d] -pyrimidine-6-carbonitrile 1b with some electrophilic and nucleophilic reagents. The structure of the target compounds were confirmed by elemental analysis and spectral data. Newly synthesized compounds showed good antimicrobial activity against microorganisms mentioned earlier.