Simplified low-cost methodology to establish, histologically process and analyze three-dimensional cancer cell spheroid arrays
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Simplified low-cost methodology to establish, histologically process and analyze three-dimensional cancer cell spheroid arrays
Different from the two-dimensional cell culture, three-dimensional (3D) model of multicellular spheroid allows cells to build interactions cell-cell / cell-matrix on the surface of cells throughout, more closely mimic microenvironments of tumors and a subpopulation cell with a certain standard morphology, differentiation and expression gen.
Since then several methodologies that involve or aggregate 3D generation cells or histological processing and analysis have appeared, but in general they are laborious, expensive and complicated to set up as a routine technique. Thus, we developed a complete methodology, detailing simple, accessible and inexpensive step by step, including 1) 3D aggregate generation cells using the technique of reduction depends; 2) provide a simple way to assess the morphological parameters of spheroids produced; followed by 3) multiples and organized histological processing, keeping some individual spheroids in an agarose tool, maintaining order is known and the position of each, similar to a tissue microarray principle; 4) until the last step, in which he allowed the simultaneous histological analysis of the composition of a few slices of round, well-organized side by side, in the same block, through conventional staining or 5) immunostaining against different molecular markers.
Therefore, the purpose of present methodology for the popularization of 3D cell culture, enabling to make these unusual techniques in basic cell biology research, after all the steps performed without the use of heavy reagents, materials or equipment. Besides bringing the apparatus simple agarose as new low cost, which allows high-throughput analysis of multiple spheroids simultaneously in an organized manner.
Simplified low-cost methodology to establish, histologically process and analyze three-dimensional cancer cell spheroid arrays
Synthesis and Evaluation of Some Novel Antimicrobial pyrimidine, pyrazole, Chromene and Tetrahydrobenzo [b] thiophene derivatives Bearing Pyrimidinthione part
Aims and objectives: A collection of novel fused pyrimidine, pyridine, pyrazole, chromene, thiophene derivatives 2-30 has been newly synthesized by using 1a, b as the starting material. Fused Pyrans show off a variety of pharmacological activities such as cancer agents [1], antimicrobial [2-4], antioxidants [5], the anti-proliferation [6], cytotoxic activity [7], the anticipated anti-tumor [8], antiparkinson [9] and anti-inflammatory [10]. Also, derivatives Pyrans famous for bacterial biofilm disruptor [11], anticonvulsants [12] and inhibtors of mycobacterium bovis [13].
Materials and methods: All melting points are measured using Block Akofler instrument and are uncorrected. IR spectrum (KBr) recorded in 5300 FTIR spectrometer (υ, cm-1). 1H-NMR were recorded on a Varian Gemini spectrometer. 1H-NMR run at 300, 400 MHz and 13 C-NMR run at 100 MHz in DMSO-d6, CDCl3 as solvent.
Chemical shifts expressed in parts per million (ppm) using tetramethylsilane (TMS) as an internal refernce. 1000 EX mass spectrometer at 70 eV. The purity of the synthesized compounds is checked by thin layer chromatography (TLC) (aluminum sheet) using n-hexane, EtOAc (9: 1, V / V, 7: 3 V / V) eluent. Elemental analysis performed by the microanalytical Research Center, Faculty of Science, and microanalytical Unit, Faculty of Pharmacy, Cairo University, Egypt.
Gastrointestinal Cancer Antigen (CA 19-9), antigen grade
Description: Cancer Antigen 15-3 MUC 1 Antigen, Host/Source: Human Milk. The purity is detected by salt extraction and delipidization following high speed centrifugation. It is tested negative for HBsAg, antibodies to HCV and HIV 1/2.
Description: Quantitativesandwich ELISA kit for measuring Human cancer antigen 27-29 (CA 27-29) in samples from serum. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human cancer antigen 27-29 (CA 27-29) in samples from serum. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Cancer Antigen 72-4 Antigen, Host/Source: Metastatic Liver. The purity is ~65% by SDS-PAGE. It is tested negative for HBsAg, antibodies to HCV and HIV 1/2.
Cancer Antigen CA 19-9 Antigen (Human adenocarcinoma cell line supernatant)
Description: Cancer Antigen 19-9 Antigen, Host/Source: Liver Carcinoma. The purity is detected by gel filtration and ion-exchange chromatography. It is tested negative for HBsAg, antibodies to HCV and HIV 1/2.
Description: Cancer Antigen 19-9 Antigen, Host/Source: Liver Carcinoma. The purity is> 90% by SDS-PAGE. It is tested negative for HBsAg, antibodies to HCV and HIV 1/2.
Cancer Antigen CEA Antigen (Human Metastatic Liver)
Description: A sandwich ELISA for quantitative measurement of Rat cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Colon Cancer Specific Antigen-3 (CCSA-3) ELISA Kit
Description: A sandwich ELISA for quantitative measurement of Goat cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Goat cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Goat cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Colon Cancer Specific Antigen 3 (CCSA-3) ELISA Kit
Description: A competitive ELISA for quantitative measurement of Rat Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
ELISA kit for Human CCSA-3 (Colon Cancer Specific Antigen-3)
Description: A sandwich ELISA kit for quantitative measurement of Human CCSA-3 (Colon Cancer Specific Antigen-3) in samples from Serum, Plasma, Cell supernatant
Description: A competitive ELISA for quantitative measurement of Human Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Colon Cancer Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human cancer associated antigen CA153 (CA15-3) purified
Description: A sandwich ELISA for quantitative measurement of Guinea pig cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Guinea pig cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Guinea pig cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Serologically defined colon cancer antigen 3 (SDCCAG3) Antibody (Biotin)
Results: A series of novel azoles and azines designed and prepared by reacting 7-amino-5- (4-chlorophenyl) – 4-phenyl-2-thioxo-2,5-dihydro – 1H pyrano- [2, 3-d] pyrimidine -6-carbonitrile 1a and 7-amino-4,5-diphenyl-2-thioxo-2,5- dihydro-1H-pyrano [2,3-d] -pyrimidine-6-carbonitrile 1b with some electrophilic and nucleophilic reagents. The structure of the target compounds were confirmed by elemental analysis and spectral data. Newly synthesized compounds showed good antimicrobial activity against microorganisms mentioned earlier.
