Simplified low-cost methodology to establish, histologically process and analyze three-dimensional cancer cell spheroid arrays

Different from the two-dimensional cell culture, three-dimensional (3D) model of multicellular spheroid allows cells to build interactions cell-cell / cell-matrix on the surface of cells throughout, more closely mimic microenvironments of tumors and a subpopulation cell with a certain standard morphology, differentiation and expression gen.

Since then several methodologies that involve or aggregate 3D generation cells or histological processing and analysis have appeared, but in general they are laborious, expensive and complicated to set up as a routine technique. Thus, we developed a complete methodology, detailing simple, accessible and inexpensive step by step, including 1) 3D aggregate generation cells using the technique of reduction depends; 2) provide a simple way to assess the morphological parameters of spheroids produced; followed by 3) multiples and organized histological processing, keeping some individual spheroids in an agarose tool, maintaining order is known and the position of each, similar to a tissue microarray principle; 4) until the last step, in which he allowed the simultaneous histological analysis of the composition of a few slices of round, well-organized side by side, in the same block, through conventional staining or 5) immunostaining against different molecular markers.

Therefore, the purpose of present methodology for the popularization of 3D cell culture, enabling to make these unusual techniques in basic cell biology research, after all the steps performed without the use of heavy reagents, materials or equipment. Besides bringing the apparatus simple agarose as new low cost, which allows high-throughput analysis of multiple spheroids simultaneously in an organized manner.

 Simplified low-cost methodology to establish, histologically process and analyze three-dimensional cancer cell spheroid arrays
Simplified low-cost methodology to establish, histologically process and analyze three-dimensional cancer cell spheroid arrays

Synthesis and Evaluation of Some Novel Antimicrobial pyrimidine, pyrazole, Chromene and Tetrahydrobenzo [b] thiophene derivatives Bearing Pyrimidinthione part

Aims and objectives: A collection of novel fused pyrimidine, pyridine, pyrazole, chromene, thiophene derivatives 2-30 has been newly synthesized by using 1a, b as the starting material. Fused Pyrans show off a variety of pharmacological activities such as cancer agents [1], antimicrobial [2-4], antioxidants [5], the anti-proliferation [6], cytotoxic activity [7], the anticipated anti-tumor [8], antiparkinson [9] and anti-inflammatory [10]. Also, derivatives Pyrans famous for bacterial biofilm disruptor [11], anticonvulsants [12] and inhibtors of mycobacterium bovis [13].


Materials and methods: All melting points are measured using Block Akofler instrument and are uncorrected. IR spectrum (KBr) recorded in 5300 FTIR spectrometer (υ, cm-1). 1H-NMR were recorded on a Varian Gemini spectrometer. 1H-NMR run at 300, 400 MHz and 13 C-NMR run at 100 MHz in DMSO-d6, CDCl3 as solvent.

Chemical shifts expressed in parts per million (ppm) using tetramethylsilane (TMS) as an internal refernce. 1000 EX mass spectrometer at 70 eV. The purity of the synthesized compounds is checked by thin layer chromatography (TLC) (aluminum sheet) using n-hexane, EtOAc (9: 1, V / V, 7: 3 V / V) eluent. Elemental analysis performed by the microanalytical Research Center, Faculty of Science, and microanalytical Unit, Faculty of Pharmacy, Cairo University, Egypt.

OPEF01469-10KU - Cancer Antigen 15-3 (CA 15-3) MUC 1 Antigen

OPEF01469-10KU 10kU
EUR 185

Gastrointestinal Cancer Antigen (CA 19-9), antigen grade

CA1991-N-10 10 KU
EUR 634.8

Cancer Antigen CA 15-3 Protein (Human Milk)

VAng-Wyb8631-inquire inquire Ask for price
Description: CA 15-3 (Breast Cancer Antigen), Antigen Grade, Cancer antigen from Human Milk, 33.0 kIU/mL.

Cancer Antigen CA 15-3 Protein (Human Fluids)

VAng-Wyb8628-inquire inquire Ask for price
Description: CA 15-3 (Breast Cancer Antigen), Cancer antigen from Human Fluids, 12.240 kU/mL.

OAEF00048-1MG - Cancer Antigen 15-3 (CA 15-3) MUC 1 Antibody

OAEF00048-1MG 1mg
EUR 1112

OAEF00234-1MG - Cancer Antigen 15-3 (CA 15-3) MUC 1 Antibody

OAEF00234-1MG 1mg
EUR 279

OAEF00235-1MG - Cancer Antigen 15-3 (CA 15-3) MUC 1 Antibody

OAEF00235-1MG 1mg
EUR 279

OAEF00905-1MG - Cancer Antigen 15-3 (CA 15-3) MUC 1 Antibody

OAEF00905-1MG 1mg
EUR 1004

OAEF00906-1MG - Cancer Antigen 15-3 (CA 15-3) MUC 1 Antibody

OAEF00906-1MG 1mg
EUR 919

OPMA04167-50KIU - Human Cancer Antigen CA15-3 Antigen Grade Protein

OPMA04167-50KIU 50KU
EUR 2999

Cancer Antigen 15-3 MUC 1 Antigen (Human Milk)

VAng-Cr3871-10kU 10 kU
EUR 505.2
Description: Cancer Antigen 15-3 MUC 1 Antigen, Host/Source: Human Milk. The purity is detected by salt extraction and delipidization following high speed centrifugation. It is tested negative for HBsAg, antibodies to HCV and HIV 1/2.

Cancer Antigen 15-3 (CA15-3) (human) ELISA Kit

K4804-100 100 assays
EUR 1000.8
Description: Sensitive, Colorimetric Assay

GWB-C9AE54-1MG - Cancer antigen 15-3 Antibody

GWB-C9AE54-1MG 1mg
EUR 389

Cancer Antigen 15-3 MUC 1 Antigen (Human Ascites Fluid)

VAng-Cr3872-10kU 10 kU
EUR 1363.2
Description: Cancer Antigen 15-3 MUC 1 Antigen, Host/Source: Human Ascites Fluid.

Ovarian Cancer Antigen (CA 125)

CA1251-N-10 10 KU
EUR 634.8

Cancer Antigen CA-125 Antigen (Human Fluids)

VAng-Wyb8639-inquire inquire Ask for price
Description: CA-125, Calibrator Grade, cancer antigen from Human Fluids, 53.26 KU/mL.

Cancer Antigen CA 19-9 Antibody

abx023798-1mg 1 mg
EUR 1053.6

Cancer Antigen CA-125 Antigen (Human Adenocarcinoma)

VAng-Wyb8642-inquire inquire Ask for price
Description: CA-125, Calibrator Grade, Cancer antigen from Human Adenocarcinoma, 500 kIU / mL.

OPEF01468-10KU - Cancer Antigen 125 (CA 125) Antigen

OPEF01468-10KU 10kU
EUR 185

OPEF01584-25KU - Cancer Antigen 125 (CA 125) Antigen

OPEF01584-25KU 25kU
EUR 377

OPEF01419-25KU - Cancer Antigen 19-9 (CA 19-9) Antigen

OPEF01419-25KU 25kU
EUR 534

OPEF01585-25KU - Cancer Antigen 19-9 (CA 19-9) Antigen

OPEF01585-25KU 25kU
EUR 343

Human Cancer Antigen 125 (CA 125) ELISA Kit

PRB-5061 96 assays
EUR 686.4

Human Cancer Antigen 125 (CA 125) ELISA Kit

PRB-5061-5 5 x 96 assays
EUR 2739.6

Cancer Antigen CA-125 Antigen (Human Fluids) (Partially Pure)

VAng-Wyb8637-inquire inquire Ask for price
Description: CA-125 (Ovarian Cancer), Partially Pure, Cancer antigen from Human Fluids, 73.90 kUnits/mL.

Cancer Antigen CA-125 Antigen (Ovarian Carcinoma Cell Line)

VAng-Wyb8641-inquire inquire Ask for price
Description: CA-125, Antigen Grade, Cancer antigen from Ovarian Carcinoma Cell Line, 84,040 Units/mL.

Cancer Antigen 125 (CA-125) (human) ELISA Kit

K4803-100 each
EUR 1038

OAEF00238-1MG - Cancer Antigen 125 (CA 125) Antibody

OAEF00238-1MG 1mg
EUR 279

OAEF00239-1MG - Cancer Antigen 125 (CA 125) Antibody

OAEF00239-1MG 1mg
EUR 279

OAEF00240-1MG - Cancer Antigen 125 (CA 125) Antibody

OAEF00240-1MG 1mg
EUR 279

OAEF00263-1MG - Cancer Antigen 125 (CA 125) Antibody

OAEF00263-1MG 1mg
EUR 279

OAEF00264-1MG - Cancer Antigen 125 (CA 125) Antibody

OAEF00264-1MG 1mg
EUR 279

OAEF00265-1MG - Cancer Antigen 125 (CA 125) Antibody

OAEF00265-1MG 1mg
EUR 279

OAEF00266-1MG - Cancer Antigen 125 (CA 125) Antibody

OAEF00266-1MG 1mg
EUR 279

Cancer Antigen CA 19-9 Antigen (Human metastatic liver carcinoma)

VAng-Wyb8635-inquire inquire Ask for price
Description: CA 19-9 (Gastrointestinal Cancer), Antigen Grade, Cancer antigen from Human metastatic liver carcinoma, 2900.0 kIU/mL.

Human CA 27-29(cancer antigen 27-29) ELISA Kit

ELK8602-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA 27-29. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA 27-29. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA 27-29, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA 27-29 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human CA 27-29(cancer antigen 27-29) ELISA Kit

ELK8602-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA 27-29. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA 27-29. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA 27-29, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA 27-29 in the samples is then determined by comparing the OD of the samples to the standard curve.

Rat cancer antigen 27-29 (CA 27-29) Elisa Kit

EK722223 96 Wells
EUR 0.64

Human cancer antigen 27-29 (CA 27-29) ELISA Kit

CSB-E13858h-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human cancer antigen 27-29 (CA 27-29) in samples from serum. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human cancer antigen 27-29 (CA 27-29) ELISA Kit

1-CSB-E13858h
  • EUR 1080.00
  • EUR 6571.20
  • EUR 3480.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human cancer antigen 27-29 (CA 27-29) in samples from serum. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human cancer antigen 27-29 (CA 27-29) ELISA Kit

SL3103Hu 96 Tests
EUR 468

Mouse cancer antigen 27-29 (CA 27-29) Elisa Kit

EK732223 96 Wells
EUR 0.86

Human cancer antigen 27-29 (CA 27-29) Elisa Kit

EK710618 96 Wells
EUR 0.11

Human cancer antigen 27-29(CA 27-29)ELISA Kit

YLA3893HU-48T 48T
EUR 435

Human cancer antigen 27-29(CA 27-29)ELISA Kit

YLA3893HU-96T 96T
EUR 562.5

Human cancer antigen 27-29 (CA 27-29) ELISA Kit

EKC32969-48T 48T
EUR 597.24

Human cancer antigen 27-29 (CA 27-29) ELISA Kit

EKC32969-5x96T 5x96T
EUR 4052.7

Human cancer antigen 27-29 (CA 27-29) ELISA Kit

EKC32969-96T 96T
EUR 853.2

Rat Cancer Antigen 19-9, CA 19-9 GENLISA ELISA

KLR1827 1 x 96 wells
EUR 341

Cancer Antigen CA 19-9 Antigen (Human adenocarcinoma cell line supernatant)

VAng-Wyb8634-inquire inquire Ask for price
Description: CA 19-9 (Gastrointestinal Cancer), Antigen Grade, Cancer antigen from Human adenocarcinoma cell line supernatant, 42,097 Units/mL.

Cancer Antigen 242 Antigen

VAng-Cr3876-10kU 10 kU
EUR 505.2
Description: Cancer Antigen 242 Antigen, Host/Source: Metastatic Liver. The purity is detected by gel filtration.

Human Cancer Antigen 27-29, CA 27-29 GENLISA ELISA

KBH4393 1 x 96 wells
EUR 286

Human Cancer Antigen 72-4, CA 72-4 GENLISA ELISA

KBH4598 1 x 96 wells
EUR 286

Cancer Antigen 50 Antigen

VAng-Cr3877-50kU 50 kU
EUR 6642
Description: Cancer Antigen 50 Antigen, Host/Source: Human Cell Culture. It is tested negative for HBsAg, antibodies to HCV and HIV 1/2.

OAEF00233-1MG - Cancer Antigen 19-9 (CA 19-9) Antibody

OAEF00233-1MG 1mg
EUR 343

Human Cancer Antigen CA15-3 Protein

abx060965-50kU 50 kU
EUR 3150

DiagNano Cancer Antigen 125 (CA 125) Conjugated Gold Nanoparticles

ACG-CAG01 5 mL
EUR 1450.8

Cancer Antigen 72-4 Antigen

VAng-Cr3878-10kU 10 kU
EUR 505.2
Description: Cancer Antigen 72-4 Antigen, Host/Source: Metastatic Liver. The purity is ~65% by SDS-PAGE. It is tested negative for HBsAg, antibodies to HCV and HIV 1/2.

Cancer Antigen 125 Antigen (Ascites Fluid)

VAng-Cr3870-10kU 10 kU
EUR 1132.8
Description: Cancer Antigen 125 Antigen, Host/Source: Ascites Fluid. The purity is ≥ 95% by SDS-PAGE.

Cancer Antigen CEA Antigen (Human Fluids)

VAng-Wyb8644-inquire inquire Ask for price
Description: Carcinoembryonic Antigen, Calibrator Grade, Cancer antigen from Human Fluids, 1.13 mg/mL.

Cancer Antigen 125 Antigen (Human Adenocarcinoma)

VAng-Cr3868-10kU 10 kU
EUR 505.2
Description: Cancer Antigen 125 Antigen, Host/Source: Human Adenocarcinoma. The purity is detected by salt extraction and chromatography.

Rabbit Prostate Cancer Antigen 3(psa3) ELISA Kit

SL0282Rb - Ask for price

Cancer Antigen 19-9 Antigen (Liver Carcinoma)

VAng-Cr3873-10kU 10 kU
EUR 472.8
Description: Cancer Antigen 19-9 Antigen, Host/Source: Liver Carcinoma. The purity is detected by gel filtration and ion-exchange chromatography. It is tested negative for HBsAg, antibodies to HCV and HIV 1/2.

Human Prostate Cancer Antigen 3, PCA3 GENLISA ELISA

KBH4340 1 x 96 wells
EUR 286

Cancer Antigen AFP Antigen (Human Cord Blood)

VAng-Wyb8624-inquire inquire Ask for price
Description: Alpha Fetoprotein (66 kDa), Cancer antigen from Human Cord Blood, 1.73 mg/mL.

OPEF01578-10KU - Cancer Antigen 242 (CA242) Antigen

OPEF01578-10KU 10kU
EUR 185

Cancer Antigen 125 Antigen (Human Adenocarcinoma) (> 90%)

VAng-Cr3869-25kU 25 kU
EUR 967.2
Description: Cancer Antigen 125 Antigen, Host/Source: Human Adenocarcinoma. The purity is> 90% by SDS-PAGE. Matched Pair: HM183, HM184, HM185, HM186.

Recombinant (E. coli) Dengue Virus Type 3 E Antigen (DENV-E), antigen grade (>95%, 15 kda, no tag)

RP-1596 50 ug
EUR 489.6

Human Cancer Antigen CA125 (Ovarian Cancer) Protein

abx061590-100kU 100 kU
EUR 1446

Pig Colon Cancer Specific Antigen-3 (CCSA-3) ELISA Kit

abx361395-96tests 96 tests
EUR 990

Cancer Antigen 19-9 Antigen (Liver Carcinoma) (> 90%)

VAng-Cr3875-25kU 25 kU
EUR 885.6
Description: Cancer Antigen 19-9 Antigen, Host/Source: Liver Carcinoma. The purity is> 90% by SDS-PAGE. It is tested negative for HBsAg, antibodies to HCV and HIV 1/2.

Cancer Antigen 125 (CA125) Antibody

E63C01601 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Cancer Antigen CEA Antigen (Human Metastatic Liver)

VAng-Wyb8645-inquire inquire Ask for price
Description: Carcinoembryonic Antigen, Antigen Grade, Cancer antigen from Human Metastatic Liver, 5.5 mg/mL.

Rabbit Colon Cancer Specific Antigen-3 (CCSA-3) ELISA Kit

abx362201-96tests 96 tests
EUR 990

Monkey Colon Cancer Specific Antigen-3 (CCSA-3) ELISA Kit

abx359619-96tests 96 tests
EUR 990

Human colon cancer-specific antigen-3,CCSA-3 ELISA kit

201-12-1721 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Chicken Colon Cancer Specific Antigen-3 (CCSA-3) ELISA Kit

abx356256-96tests 96 tests
EUR 990

Human colon cancer-specific antigen-3(CCSA-3)ELISA Kit

GA-E1737HM-48T 48T
EUR 346.8

Human colon cancer-specific antigen-3(CCSA-3)ELISA Kit

GA-E1737HM-96T 96T
EUR 559.2

Human colon cancer-specific antigen-3,CCSA-3 ELISA Kit

SL0494Hu 96 Tests
EUR 468

Human colon cancer-specific antigen-3,CCSA-3 ELISA kit

YLA0511HU-48T 48T
EUR 435

Human colon cancer-specific antigen-3,CCSA-3 ELISA kit

YLA0511HU-96T 96T
EUR 562.5

Human colon cancer-specific antigen-3(CCSA-3)ELISA Kit

QY-E03946 96T
EUR 433.2

Serologically Defined Colon Cancer Antigen 3 (SDCCAG3) Antibody

abx237665-100ug 100 ug
EUR 610.8

Serologically defined colon cancer antigen 3 (SDCCAG3) Antibody

20-abx213359
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Serologically defined colon cancer antigen 3 (SDCCAG3) Antibody

20-abx213492
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Serologically Defined Colon Cancer Antigen 3 (SDCCAG3) Antibody

20-abx115457
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Serologically defined colon cancer antigen 3 (SDCCAG3) Antibody

20-abx334226
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Serologically Defined Colon Cancer Antigen 3 (SDCCAG3) Antibody

20-abx005975
  • EUR 493.20
  • EUR 710.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 50 ul

Inactivated MuV Grade 3 Antigen

VAng-Lsx0411-inquire inquire Ask for price
Description: MuV grade 3, native protein.

Inactivated RuV Grade 3 Antigen

VAng-Lsx0476-1mL 1 mL
EUR 474
Description: RuV Grade 3, natural protein.

Inactivated CMV Grade 3 Antigen

VAng-Lsx0038-1mg 1 mg
EUR 2503.2
Description: CMV Grade 3, natural protein.

Human Colon Cancer Specific Antigen 3 (CCSA-3) ELISA Kit

abx354339-96tests 96 tests
EUR 848.4

CEA (Cancer antigen) ELISA test

9 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CEA (Cancer antigen)

CA50 (Cancer antigen) ELISA test

10 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CA50 (Cancer antigen)

Human Colon Cancer-specific Antigen-3, CCSA-3 GENLISA ELISA

KBH1720 1 x 96 wells
EUR 286

Cancer Antigen AFP Antigen (Human hepatocellular carcinoma)

VAng-Wyb8625-inquire inquire Ask for price
Description: Alpha Fetoprotein, Antigen Grade, Cancer antigen from human hepatocellular carcinoma, 2.93 mg/mL.

CA199 (Cancer antigen) ELISA test

13 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CA199 (Cancer antigen)

CA153 (Cancer antigen) ELISA test

12 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CA153 (Cancer antigen)

CA125 (Cancer antigen) ELISA test

11 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CA125 (Cancer antigen)

Cancer Antigen CA19-9 Antibody

abx020532-1mg 1 mg
EUR 1296

Cancer Antigen CA19-9 Antibody

abx020533-1mg 1 mg
EUR 718.8

Cancer Antigen CA19-9 Antibody

abx020534-1mg 1 mg
EUR 718.8

Cancer Antigen CA19-9 Antibody

abx020535-1mg 1 mg
EUR 718.8

ELISA kit for Human CCSA-3 (Colon Cancer Specific Antigen-3)

E-EL-H0794 1 plate of 96 wells
EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Human CCSA-3 (Colon Cancer Specific Antigen-3) in samples from Serum, Plasma, Cell supernatant

Inactivated Rotavirus Grade 3 Antigen

VAng-Lsx0469-inquire inquire Ask for price
Description: Rotavirus Grade 3, native virus.

OPEF01577-10KU - Cancer Antigen 72-4 (CA72-4) Antigen

OPEF01577-10KU 10kU
EUR 185

Cancer Antigen 19-9 Antigen (Liver Carcinoma) (High purity)

VAng-Cr3874-25kU 25 kU
EUR 1033.2
Description: Cancer Antigen 19-9 Antigen, Host/Source: Liver Carcinoma.

Rat cancer antigen 27-29 ELISA kit

E02C1276-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat cancer antigen 27-29 ELISA kit

E02C1276-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat cancer antigen 27-29 ELISA kit

E02C1276-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog cancer antigen 27-29 ELISA kit

E08C1276-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog cancer antigen 27-29 ELISA kit

E08C1276-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog cancer antigen 27-29 ELISA kit

E08C1276-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig cancer antigen 27-29 ELISA kit

E07C1276-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig cancer antigen 27-29 ELISA kit

E07C1276-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig cancer antigen 27-29 ELISA kit

E07C1276-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat cancer antigen 27-29 ELISA Kit

E01A11532 96T
EUR 700
Description: ELISA

Human Cancer Antigen 125 (CA125) Protein

abx061598-250kU 250 kU
EUR 1195.2

Cancer Antigen 125 (CA125) Antibody-HRP

E63C01602 100μg/100μl
EUR 275
Description: Available in various conjugation types.

Goat cancer antigen 27-29 ELISA kit

E06C1276-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat cancer antigen 27-29 ELISA kit

E06C1276-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat cancer antigen 27-29 ELISA kit

E06C1276-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat cancer antigen 27-29 ELISA Kit

E01A46444 96T
EUR 700
Description: ELISA

Human cancer associated antigen CA153 (CA15-3) purified

CA153-N-10 10 KU
EUR 781.2

Serologically defined colon cancer antigen 3 (SDCCAG3) Antibody (HRP)

20-abx337646
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Serologically defined colon cancer antigen 3 (SDCCAG3) Antibody (FITC)

20-abx337647
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Canine cancer antigen 27-29 ELISA Kit

E01A63877 96T
EUR 700
Description: ELISA

Human cancer antigen 27-29 ELISA kit

E01C1276-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human cancer antigen 27-29 ELISA kit

E01C1276-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Human cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human cancer antigen 27-29 ELISA kit

E01C1276-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Human cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse cancer antigen 27-29 ELISA kit

E03C1276-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse cancer antigen 27-29 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.


Results: A series of novel azoles and azines designed and prepared by reacting 7-amino-5- (4-chlorophenyl) – 4-phenyl-2-thioxo-2,5-dihydro – 1H pyrano- [2, 3-d] pyrimidine -6-carbonitrile 1a and 7-amino-4,5-diphenyl-2-thioxo-2,5- dihydro-1H-pyrano [2,3-d] -pyrimidine-6-carbonitrile 1b with some electrophilic and nucleophilic reagents. The structure of the target compounds were confirmed by elemental analysis and spectral data. Newly synthesized compounds showed good antimicrobial activity against microorganisms mentioned earlier.